Volume 26, Issue 1, Pages 280-288 (January 2018) Absence of Replication-Competent Lentivirus in the Clinic: Analysis of Infused T Cell Products  Kenneth Cornetta, Lisa Duffy, Cameron J. Turtle, Michael Jensen, Stephen Forman, Gwendolyn Binder-Scholl, Terry Fry, Anne Chew, David G. Maloney, Carl H. June  Molecular Therapy  Volume 26, Issue 1, Pages 280-288 (January 2018) DOI: 10.1016/j.ymthe.2017.09.008 Copyright © 2017 The American Society of Gene and Cell Therapy Terms and Conditions

Figure 1 Schematic Representation of the RCL Assay T cell products are incubated with C8166-45 cells at a ratio of 1:5. Over half of test articles submitted contained approximately 1 × 108 T cells and were divided into twenty-five 300-cm2 flasks. Cultures were passed a minimum of 5 passages using increasingly smaller vessels. After 3 weeks, cells were placed in fresh media, and conditioned media were harvested after 24 hr. Media from all amplification cultures are pooled, and two aliquots are then incubated with naive C8166-45 cells. After 7 days, culture media were analyzed for p24 antigen by ELISA and either the psi-gag PCR assay or PERT. The figure depicts amplification of a low-titer RCL present in the test article. Molecular Therapy 2018 26, 280-288DOI: (10.1016/j.ymthe.2017.09.008) Copyright © 2017 The American Society of Gene and Cell Therapy Terms and Conditions