Gene expression in women conceiving spontaneously over the age of 45 years  Yuval Gielchinsky, M.D., Yoel Bogoch, M.Sc., Gideon Rechavi, M.D., Ph.D., Jasmine Jacob-Hirsch, M.Sc., Ninette Amariglio, Ph.D., David Shveiky, M.D., Michal Linial, Ph.D., Neri Laufer, M.D.  Fertility and Sterility  Volume 89, Issue 6, Pages 1641-1650 (June 2008) DOI: 10.1016/j.fertnstert.2007.06.058 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Flowchart of the analysis. Fertility and Sterility 2008 89, 1641-1650DOI: (10.1016/j.fertnstert.2007.06.058) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (A) Cluster analysis of peripheral blood mononuclear cells (PMNC) isolated from the senior fertile group of women who conceived after the age of 45 years, (n = 8) and from controls (n = 6). Each column represents the gene expression of one individual woman, and each row represents a single gene. Red squares represent higher than median level gene expression (up-regulation), and green squares represent lower than median level gene expression (down-regulation). A total of 716 probes (associated with 671 genes) that were differentially expressed between SF women and the control group are shown. (B) Division of the 671 differentially expressed genes according to Gene Ontology (GO) annotation for cellular processes. The large fraction of unclassified genes was excluded from the chart. Fertility and Sterility 2008 89, 1641-1650DOI: (10.1016/j.fertnstert.2007.06.058) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Genes related to apoptosis, ubiquitination, and energy production pathways. Genes that showed overexpression in senior fertile (SF) cases relative to controls are in bold letters; the rest of the genes were underexpressed in SF relative to controls. Genes shared between the functional categories are colored as follows: genes shared in all three categories are purple; genes shared between apoptosis and ubiquitination are blue; genes shared between the apoptosis and energy production categories are red; genes shared between the ubiquitination and energy production categories are green. An asterisk denotes a reported human aging gene. In brackets is the distinction value, which indicates the difference in expression of the SF and control groups. Fertility and Sterility 2008 89, 1641-1650DOI: (10.1016/j.fertnstert.2007.06.058) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions