A, Western blot analysis of fetuin-A in AGA (lanes 1–5 and 10–13) and IUGR (lanes 6–9, 14, and 15) UC plasma samples. A, Western blot analysis of fetuin-A.

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A, Western blot analysis of fetuin-A in AGA (lanes 1–5 and 10–13) and IUGR (lanes 6–9, 14, and 15) UC plasma samples. A, Western blot analysis of fetuin-A in AGA (lanes 1–5 and 10–13) and IUGR (lanes 6–9, 14, and 15) UC plasma samples. Equal protein amounts (1 μg) of UC plasma samples from AGA and IUGR neonates were separated by 10% SDS-PAGE under reducing conditions and immunoblotted with antibody against human fetuin-A. Arrows point to the IUGR-related fetuin-A isoform. Lane 7 represents one of the IUGR UC plasma samples that did not present the IUGR-specific Al/Bl row of spots in the 2-DE analysis. B–E, 2-D Western blot analysis of fetuin-A confirming the identity of group A as the heavy chain and group B as the single chain form of fetuin-A. Equal protein amounts (50 μg) of UC plasma samples from AGA (B and D) and IUGR (C and E) neonates were separated on pH 4–7 linear IPG strips. The second dimension was carried out using 10% SDS-PAGE. Plasma samples were treated under either reducing (B and C) or non-reducing conditions (D and E). After transfer, membranes were immunoblotted with antibody against human fetuin-A. Numbers on the top of the panels indicate the pI; molecular mass markers are shown on the left. Panagiotis M. Karamessinis et al. Mol Cell Proteomics 2008;7:591-599 © 2008 The American Society for Biochemistry and Molecular Biology