Analysis of DNA from degraded tissue

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Presentation transcript:

Analysis of DNA from degraded tissue Patiya Phengon, Sirinan Wongwiggarn, Nathinee Panvisavas  Forensic Science International: Genetics Supplement Series  Volume 1, Issue 1, Pages 439-441 (August 2008) DOI: 10.1016/j.fsigss.2007.11.012 Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Pork tissue samples in various conditions. (a) Fresh pork meat, samples left for 6 days (b) to air dry, (c) in sea water and (d) tap water. Forensic Science International: Genetics Supplement Series 2008 1, 439-441DOI: (10.1016/j.fsigss.2007.11.012) Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Map of β-actin primers. Combinations of these primers generate DNA fragments sizes of 289, 366, 211 and 289bp. Forensic Science International: Genetics Supplement Series 2008 1, 439-441DOI: (10.1016/j.fsigss.2007.11.012) Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Two percent (w/v) agarose/TBE gels showing the 211-, 289- and 366-bp β-actin fragments amplified from tissue sample up to 8 days (A). (B) The 289-bp β-actin fragments were amplified from one of two air-dried duplicates at day 2 and 4. The 289-bp β-actin fragments were not detected in samples that were for left air-drying for more than 4 days. Forensic Science International: Genetics Supplement Series 2008 1, 439-441DOI: (10.1016/j.fsigss.2007.11.012) Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions