Fig S1 Figure S1. Frequent mutation in clear cell renal cell carcinoma. Figure S1 shows the top 20 genes ordered by the occurrence of mutations from COSMIC.

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Fig S1 Figure S1. Frequent mutation in clear cell renal cell carcinoma. Figure S1 shows the top 20 genes ordered by the occurrence of mutations from COSMIC database (downloaded on March, 2015). ~4% sample of total ccRCC patients have p53 mutation.

Fig S2 a CAKI-1 b CAKI-1 TGase2 siRNA #1+2 Control siRNA TGase2 siRNA #1 TGase2 siRNA #2 Control siRNA p53 siRNA #1 p53 siRNA #2 p53 siRNA #1+2 TGase2 -75 kDa p53 -50 p53 -50 kDa HDM2 -60 β-actin -40 kDa TGase2 -75 β-actin -40 c CAKI-1 d CAKI-1 Control siRNA GK921 p53 siRNA p53 siRNA +GK921 HDM2 siRNA #1+2 Control siRNA HDM2 siRNA #1 HDM2 siRNA #2 HDM2 -60 kDa TGase2 -75 kDa p53 -50 kDa p-p53 -50 kDa β-actin -40 kDa p53 -50 kDa Cleaved -PARP -85 kDa β-actin -40 kDa Figure S2. TGase 2 and HDM2 suppressed p53. (a, b and c) CAKI-1 cells were transfected with siRNA targeting TG2, HDM2 and p53 separately. (d) CAKI-1 cells were transfected with p53 siRNA for 48 h. During transfection, GK921 1 μM treated control siRNA or p53 siRNA transfected cell for 24 h.

Fig S3 a b DAPI FITC Merge Control Nutlin-3a ★★★ ★★ CAKI-1 ACHN 10 μm Figure S3. (a) ACHN and CAKI-1 cells were treated with Nutlin-3a 5μM and stained with TUNEL and DAPI . Scale bar= 20 μm. (b) The percentage of TUNEL positive cells in each case was counted. The graphs are representative of three independent experiments. Each data is from three independent experiments, ± SD (n = 3). p**<0.01, p***<0.001. All experiments were conducted under starvation with EBSS for 6 h