Physiologic Effects Of Extracellular Superoxide Dismutase Transgene Overexpression On Myocardial Function After Ischemia And Reperfusion Injury Edward.

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Presentation transcript:

Physiologic Effects Of Extracellular Superoxide Dismutase Transgene Overexpression On Myocardial Function After Ischemia And Reperfusion Injury Edward P. Chen, MD, Hartmuth B. Bittner, MD,PhD, R.Duane Davis, MD, Peter Van Trigt, MD, Rodney J. Folz, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 115, Issue 2, Pages 450-459 (February 1998) DOI: 10.1016/S0022-5223(98)70289-2 Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 1 The isolated work-performing murine heart preparation. The key features of this system include jacketed water baths, reservoirs, and water lines to minimize heat loss; an in-line ultrasonic flow probe to measure cardiac output; aortic and left atrial (LA) micromanometers; and a computerized data acquisition and analysis system. The Journal of Thoracic and Cardiovascular Surgery 1998 115, 450-459DOI: (10.1016/S0022-5223(98)70289-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

The Journal of Thoracic and Cardiovascular Surgery 1998 115, 450-459DOI: (10.1016/S0022-5223(98)70289-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 2 Reverse-transcriptase polymerase chain reaction analysis. Using this technique, myocardial expression of the 174 base pair (bp) human extracellular superoxide dismutase messenger RNA was found to be present in transgenic mice (E) and absent in control (C) mice. The Journal of Thoracic and Cardiovascular Surgery 1998 115, 450-459DOI: (10.1016/S0022-5223(98)70289-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 3 The preischemic and postischemic cardiac outputs (CO) of both experimental groups are plotted against gradually increasing preload levels at a constant afterload of 55 mm Hg. Before ischemia, no significant differences were observed in the CO of CTL mice compared with EC-SOD mice. Significant decreases in CO were observed for both groups after normothermic ischemia; however, this decrease was significantly larger in CTL hearts compared with EC-SOD hearts. *Denotes p < 0.05 versus EC-SOD-Pre. †Denotes p < 0.05 versus CTL-Post. ‡Denotes p < 0.01 versus CTL-Pre. The Journal of Thoracic and Cardiovascular Surgery 1998 115, 450-459DOI: (10.1016/S0022-5223(98)70289-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 4 The differences in the average contractility (dP/dt) between the two experimental groups before and after normothermic ischemia. The average dP/dt decreased significantly in both EC-SOD and CTL hearts after normothermic ischemia. *Denotes p < 0.001 versus CTL, Pre-ischemia. †Denotes p = 0.04 versus EC-SOD, Pre-ischemia. The Journal of Thoracic and Cardiovascular Surgery 1998 115, 450-459DOI: (10.1016/S0022-5223(98)70289-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 5 The differences in the average stroke work (SW) between the two experimental groups before and after normothermic ischemia. The decrease in the average SW of CTL hearts after ischemia was significantly greater compared with the decrease in the average SW of EC-SOD hearts. *Denotes p = 0.003 versus CTL, Pre-ischemia. †Denotes p = 0.005 versus EC-SOD, Pre-ischemia. ‡Denotes p = 0.02 versus CTL, Post-ischemia. The Journal of Thoracic and Cardiovascular Surgery 1998 115, 450-459DOI: (10.1016/S0022-5223(98)70289-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 6 The differences in the average heart rates (HR) between the two experimental groups before and after normothermic ischemia. The average HR decreased significantly in both EC-SOD and CTL hearts after normothermic ischemia. *Denotes p = 0.03 versus CTL, Pre-ischemia. †Denotes p = 0.03 versus EC-SOD, Pre-ischemia. The Journal of Thoracic and Cardiovascular Surgery 1998 115, 450-459DOI: (10.1016/S0022-5223(98)70289-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 7 The differences in the average stroke volume (SV) between the two experimental groups before and after normothermic ischemia. The decrease in the average SV of CTL hearts after ischemia was significantly greater compared with the decrease in the average SV of EC-SOD hearts. *Denotes p = 0.002 versus CTL, Pre-ischemia. †Denotes p = 0.006 versus EC-SOD, Pre-ischemia. ‡Denotes p = 0.03 versus CTL, Post-ischemia. The Journal of Thoracic and Cardiovascular Surgery 1998 115, 450-459DOI: (10.1016/S0022-5223(98)70289-2) Copyright © 1998 Mosby, Inc. Terms and Conditions