Dynamic Pluripotent Stem Cell States and Their Applications Jun Wu, Juan Carlos Izpisua Belmonte  Cell Stem Cell  Volume 17, Issue 5, Pages 509-525 (November 2015) DOI: 10.1016/j.stem.2015.10.009 Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 1 Spatiotemporally Distinct PSC States PSCs showing different timing and spatial properties have been isolated from the early mouse embryos. Top: illustrations of mouse embryos at different stages of early development. Embryonic cells with totipotent or pluripotent potentials are indicated. Bottom: in vitro cultured cells showing functional features resembling in vivo embryonic cells and categorized into different pluripotent states accordingly. Cell morphologies of mESCs, EpiSCs, and rsEpiSCs are shown. Cell Stem Cell 2015 17, 509-525DOI: (10.1016/j.stem.2015.10.009) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 2 Interspecies Chimeric Complementation By genetically altering the host embryos (e.g., Pdx1−/−), developmental programs specific for certain lineages and organs can be disabled. Totipotent, pluripotent, or multi-potent stem cells from one species can potentially be used to rescue the organ defects of the host species at different time points during development. And as a result, organs enriched with cells derived from donor stem cells are generated in a xeno environment. Cell Stem Cell 2015 17, 509-525DOI: (10.1016/j.stem.2015.10.009) Copyright © 2015 Elsevier Inc. Terms and Conditions