Relative amount of nuclear β-catenin

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Relative amount of nuclear β-catenin 2016/1/27 山崎 A β-catenin/ Alexa568 DAPI EYFP-NLS-actin merge b-catenin/ Alexa488 mCherry- NLS-actin DAPI merge B DAPI EYFP β-catenin merge EYFP EYFP-actin EYFP-NLS-actin Relative amount of nuclear β-catenin -serum C ** Fig. 1

Relative amount of nuclear β-catenin ** Relative amount of nuclear β-catenin ** Relative amount of nuclear β-catenin -XAV939 / +serum +XAV939 / +serum B DAPI EYFP β-catenin merge HaCaT cell EYFP-actin EYFP-NLS-actin C Relative amount of nuclear β-catenin +serum ** Fig. 2

* ** * * Fig. 3 A B C Relative luciferase activity Relative expression level * Relative increase of cells * C Fig. 3

Fold enrichment A CCND1 * * Fold enrichment B OCT4 ** * Fig. 4

** ** * * * Fig. 5 A B // OCT4 GAPDH CCND1 β-catenin binding site -1335 bp -110 bp ±0 bp +890 bp ±0 bp +2100 bp SP1 site TSS exon4 TSS exon ** ** Fold enrichment * * EYFP-actin EYFP-NLS-actin EYFP-NLS-G13R-actin EYFP-actin EYFP-NLS-actin EYFP-NLS-G13R-actin EYFP-actin EYFP-NLS-actin EYFP-NLS-G13R-actin EYFP-actin EYFP-NLS-actin EYFP-NLS-G13R-actin EYFP-actin EYFP-NLS-actin EYFP-NLS-G13R-actin EYFP-actin EYFP-NLS-actin EYFP-NLS-G13R-actin // B Fold enrichment CCND1 * Fig. 5