Validation of p53- and miRNA-mediated down-regulation

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Validation of p53- and miRNA-mediated down-regulation Validation of p53- and miRNA-mediated down-regulation. qPCR analysis of the indicated mRNAs after (A) addition of DOX to SW480/pRTR-p53-VSV cells for the indicated periods of time, (B) after treatment of HCT116 p53+/+ and p53−/− cells with Nutlin or Etoposide for 48 h. Validation of p53- and miRNA-mediated down-regulation. qPCR analysis of the indicated mRNAs after (A) addition of DOX to SW480/pRTR-p53-VSV cells for the indicated periods of time, (B) after treatment of HCT116 p53+/+ and p53−/− cells with Nutlin or Etoposide for 48 h. C, Dual reporter assays of H1299 cells cotransfected with the indicated miRNA mimics or controls and the indicated 3′-UTR constructs. D, Details of the mutant 3′-UTR reporter constructs. SM = seed-match. Results in (A), (B), and (C) represent the mean ± S.D. (n = 3). Sabine Hünten et al. Mol Cell Proteomics 2015;14:2609-2629 © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.