Testing the effectiveness of the three-step peptide fractionation method.A, μLC mass chromatograms of SCX fractions for an acidic FFE fraction. Testing.

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Testing the effectiveness of the three-step peptide fractionation method.A, μLC mass chromatograms of SCX fractions for an acidic FFE fraction. Testing the effectiveness of the three-step peptide fractionation method.A, μLC mass chromatograms of SCX fractions for an acidic FFE fraction. B, μLC mass chromatograms of SCX fractions for a basic FFE fraction. C, number of peptides (top) and resulting proteins (bottom) identified from the same FFE fraction analyzed using either the two-step (FFE-μLC) or three-step fractionation (FFE-SCX-μLC) method. Hongwei Xie et al. Mol Cell Proteomics 2008;7:486-498 © 2008 The American Society for Biochemistry and Molecular Biology