In situ tissue regeneration using a novel tissue-engineered, small-caliber vascular graft without cell seeding Takenori Yokota, MD, Hajime Ichikawa,

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Presentation transcript:

In situ tissue regeneration using a novel tissue-engineered, small-caliber vascular graft without cell seeding Takenori Yokota, MD, Hajime Ichikawa, MD, PhD, Goro Matsumiya, MD, PhD, Toru Kuratani, MD, PhD, Taichi Sakaguchi, MD, PhD, Shigemitsu Iwai, MD, PhD, Yukitoshi Shirakawa, MD, PhD, Kei Torikai, MD, PhD, Atsuhiro Saito, PhD, Eiichiro Uchimura, PhD, Naomasa Kawaguchi, PhD, Nariaki Matsuura, MD, PhD, Yoshiki Sawa, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 136, Issue 4, Pages 900-907 (October 2008) DOI: 10.1016/j.jtcvs.2008.02.058 Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 The newly designed small-diameter vascular graft (A, B) was fabricated using a biodegradable woven polymer tube constructed in a plain weave pattern using a double-layered thread with PLLA fibers as the core and PGA fibers as the sheath. Scanning electron micrograph of the double-layered thread (C). The graft surface shows the porous 3-dimensional structure (D) and interconnected pore structure after incorporation of the collagen microsponge (E) (magnification, 100×). The Journal of Thoracic and Cardiovascular Surgery 2008 136, 900-907DOI: (10.1016/j.jtcvs.2008.02.058) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 The number of cells of NIH3T3 (A) and human umbilical vascular endothelial cells (B) cultured for 3 days in vitro on TEVGs with/without the collagen microsponge. Scanning electron micrographs of the same samples with NIH3T3 (C) and human umbilical vascular endothelial cells (D) cultured for 3 days in vitro. HUVEC, Human umbilical vascular endothelial cell. The Journal of Thoracic and Cardiovascular Surgery 2008 136, 900-907DOI: (10.1016/j.jtcvs.2008.02.058) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Macroscopic appearance of TEVGs 2 (A), 4 (B), 6 (C), and 12 (D) months after implantation and of the ePTFE graft 12 months after implantation (E). The scale bar represents 10 mm. The Journal of Thoracic and Cardiovascular Surgery 2008 136, 900-907DOI: (10.1016/j.jtcvs.2008.02.058) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Scanning electron micrograph of the luminal surfaces of TEVGs 2 (A), 4 (B), 6 (C), and 12 (D) months after implantation, compared with that of the ePTFE graft 12 months after implantation (E) (magnification, 500×). The Journal of Thoracic and Cardiovascular Surgery 2008 136, 900-907DOI: (10.1016/j.jtcvs.2008.02.058) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 Double staining with hematoxylin-eosin and Victoria blue stain (A), immunohistochemical double staining with von Willebrand factor and α-smooth muscle actin (B), and elastica van Gieson (C) of the PGA/PLLA tube graft 2, 4, 6, and 12 months after implantation, and those of the ePTFE graft 12 months after implantation. The scale bar represents 100 μm. H&E, Hematoxylin-eosin; vWF, von Willebrand factor; SMA, smooth muscle actin; EVG, elastica van Gieson; ePTFE, expanded polytetrafluoroethylene. The Journal of Thoracic and Cardiovascular Surgery 2008 136, 900-907DOI: (10.1016/j.jtcvs.2008.02.058) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 6 The regenerated neointimal and medial wall thickness of the proximal, middle, and distal portions of TEVGs 2, 4, 6, and 12 months after implantation. Error bars indicate standard deviation. The wall thickness increased significantly over time from 2 to 6 months (∗P < .05). The Journal of Thoracic and Cardiovascular Surgery 2008 136, 900-907DOI: (10.1016/j.jtcvs.2008.02.058) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 7 Biochemical analysis of collagen content in the neotissue of TEVGs 2, 4, 6, and 12 months after implantation and that of the native carotid artery (∗P < .05). The Journal of Thoracic and Cardiovascular Surgery 2008 136, 900-907DOI: (10.1016/j.jtcvs.2008.02.058) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 8 Biomechanical analysis showing tensile modulus and tensile strength of TEVGs at preimplantation stage and 2, 4, 6, and 12 months after implantation, and those of the native carotid artery (∗P < .05). MPa, Megapascal. The Journal of Thoracic and Cardiovascular Surgery 2008 136, 900-907DOI: (10.1016/j.jtcvs.2008.02.058) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions