Marcel Scheinman, MD, Enrico Ascher, MD, Gabriel S

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p53 gene transfer to the injured rat carotid artery decreases neointimal formation Marcel Scheinman, MD, Enrico Ascher, MD, Gabriel S. Levi, MD, Anil Hingorani, MD, Djamshid Shirazian, PhD, Prem Seth, PhD Journal of Vascular Surgery Volume 29, Issue 2, Pages 360-369 (February 1999) DOI: 10.1016/S0741-5214(99)70389-7 Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Western blot analysis of p53 expression in rat carotid arteries 4 days after balloon injury. Positive detection is shown on lanes 1, 2, and 3 (AdWTp53, 8 × 109 pfu/mL, 1.6 × 1010 pfu/mL, and 8 × 1010 pfu/mL, respectively), whereas lanes 4 (vehicle) and 5 (AdNull) show an absence of p53 bands. Journal of Vascular Surgery 1999 29, 360-369DOI: (10.1016/S0741-5214(99)70389-7) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Inhibition of neointima formation by AdWTp53 gene transfer 14 days after balloon injury of the rat carotid artery. The data are shown as mean ± SEM. The AdWTp53 groups have 3 concentrations: #1, 8 × 1010 pfu/mL; #2, 1.6 × 1010 pfu/mL; and #3, 8 × 109 pfu/mL. Group 1 has 8 animals, whereas the remaining groups have 6 animals in each. *P < .001 versus controls (vehicle + AdNull, 8 × 1010 pfu/mL). **P = .003 versus AdWTp53 #1. ***P = .002 versus AdWTp53 #1 by Student's t test. Journal of Vascular Surgery 1999 29, 360-369DOI: (10.1016/S0741-5214(99)70389-7) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Inhibition of neointima formation by AdWTp53. Representative photomicrographs of cross sections from rat carotid arteries 14 days after surgery. Cross sections were taken from the center of each vessel. The neointima is demarcated between arrows. (A) AdNull. (B) AdWTp53, 8 × 1010 pfu/mL; near-total arrest of neointimal formation. (C) Vehicle. (D) AdWTp53, 1.6 × 1010 pfu/mL. (E) AdWTp53, 8 × 1010 pfu/mL; 1 layer of cells is shown. The photomicrographs illustrate the effect of AdWTp53 on decreasing neointima formation. Hematoxylin-eosin. Magnification: A and B, ×40; C—E, ×100. Journal of Vascular Surgery 1999 29, 360-369DOI: (10.1016/S0741-5214(99)70389-7) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Immunohistochemistry for p53. (a) Negative staining. (b) Positive staining. A = adventitia; M = media; NI = neointima; I = intima. Arrow shows stained nucleus. Mayer's hematoxylin, magnification ×400. Journal of Vascular Surgery 1999 29, 360-369DOI: (10.1016/S0741-5214(99)70389-7) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Immunohistochemistry for von Willebrand factor. Positive staining (arrows) observed in the endothelium of rat carotid arteries after balloon injury and p53 gene transfer. (A) AdWTp53, 8 × 1010 pfu/mL. (B) AdWTp53, 1.6 × 1010 pfu/mL. Hematoxylin, magnification ×400. Journal of Vascular Surgery 1999 29, 360-369DOI: (10.1016/S0741-5214(99)70389-7) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions