The FLOWERING LOCUS T/TERMINAL FLOWER 1 Gene Family: Functional Evolution and Molecular Mechanisms  Daniel P. Wickland, Yoshie Hanzawa  Molecular Plant  Volume 8, Issue 7, Pages 983-997 (July 2015) DOI: 10.1016/j.molp.2015.01.007 Copyright © 2015 The Author Terms and Conditions

Figure 1 Phylogenetic Tree of the FT/TFL1 Protein Family. Maximum likelihood phylogenetic tree of the FT/TFL1 protein family in 50 plant species characterized by transgenic approaches. The tree was constructed using the Maximum Likelihood method in MEGA6 software (Tamura et al., 2013). Bootstrap values >30% for 1000 re-samplings are shown on branches. Blue and red branches indicate FT-like and TFL1-like proteins, respectively. Protein names in brown indicate FT-like proteins with repressor function. Arabidopsis proteins are shown in bold. Molecular Plant 2015 8, 983-997DOI: (10.1016/j.molp.2015.01.007) Copyright © 2015 The Author Terms and Conditions

Figure 2 Amino Acid Alignment of Inducer FTs and Repressor FTs at the Conserved External Loop in Exon 4. Alignment of amino acid sequences of FT homologs at the conserved position 85 (exon 2) and segment B (positions 128–141, exon 4) from Arabidopsis (Kardailsky et al., 1999; Kobayashi et al., 1999), onion (Lee et al., 2013), sugar beet (Pin et al., 2010), longan (Winterhagen et al., 2013), soybean (Kong et al., 2010; Nan et al., 2014; Zhai et al., 2014), sunflower (Blackman et al., 2010), tobacco (Harig et al., 2012), and sugarcane (Coelho et al., 2014). FT-/TFL1-like proteins from Norway spruce are also included (Karlgren et al., 2011). Red asterisks indicate repressor function characterized in a gene’s native species and blue asterisks indicate repressor function characterized in transgenic Arabidopsis. Amino acid positions indicated are based on the Arabidopsis FT protein sequence. All inducer FTs (except GmFT5a) contain tyrosine at position 134, while repressor FTs (except FT-/TFL1-like PaFTL1 and PaFTL2) contain non-tyrosine amino acids. In addition, all inducer FTs contain tryptophan at position 138, while repressor FTs (except DlFT2 and GmFT4) contain non-tryptophan amino acids. Protein sequences with the following IDs were obtained from The Arabidopsis Information Resource (TAIR), GenBank, and Phytozome: AT1G65480.1 (AtFT), AT5G03840.1 (AtTFL1), AGZ20207.1 (AcFT1), AGZ20210.1 (AcFT4), ADM92608.1 (BvFT1), ADM92610.1 (BvFT2), AEZ63949.1 (DlFT1), AEZ63950.1 (DlFT2), BAJ33491.1 (GmFT2a), Glyma08g47810.1 (GmFT4), BAJ33494.1 (GmFT5a), ADF32943.1 (HaFT1), ADF32945.1 (HaFT4), AFS17369.1 (NtFT1), AFS17370.1 (NtFT2), AFS17371.1 (NtFT3), AFS17372.1 (NtFT4), AEH59567.1 (PaFTL1), ABQ85553.1 (PaFTL2), AHZ46121.1 (ScFT1). Molecular Plant 2015 8, 983-997DOI: (10.1016/j.molp.2015.01.007) Copyright © 2015 The Author Terms and Conditions