Leukocytes in human PDAC

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Leukocytes in human PDAC Leukocytes in human PDAC. A, relative CD20 and Ig mRNA expression in human pancreatic ductal adenocarcinoma (AdenoCa; n = 33), IPMN (n = 5), and islet cell carcinomas (Islet Cell Ca; n = 6), as compared with healthy pancreas tissue (n = 17) assessed by Affy... Leukocytes in human PDAC. A, relative CD20 and Ig mRNA expression in human pancreatic ductal adenocarcinoma (AdenoCa; n = 33), IPMN (n = 5), and islet cell carcinomas (Islet Cell Ca; n = 6), as compared with healthy pancreas tissue (n = 17) assessed by Affymatrix Human U133 Plus 2 microarrays. Data are represented as box-and-whisker plots depicting median fold-change value compared with normal tissue, displaying the first and third quartiles at the end of each box, with the maximum and minimum at the ends of the whiskers. Statistical significance was determined via the Wilcoxon rank-sum test with *, P < 0.05; ***, P < 0.001. B, FACS quantitation of CD45+ cells as a percentage of viable cells from pancreas tissues reflecting healthy (H) pancreata (n = 6), pancreas tissue adjacent to PDAC (Adj; n = 13), PDAC tumor margins (M; n = 4) and tumor centers (Ctr; n = 23) from patients who had not received prior chemotherapy (−CTX), and PDACs (n = 3) from patients treated with standard-of-care CTX (+CTX). Each data point reflects an individual piece of tissue. C, leukocyte complexity of healthy human pancreas (n = 6), and pancreas tissue isolated from patients with PDAC evaluated by polychromatic flow cytometry of single-cell suspensions and evaluated for expression of the lineage markers shown. Data represent mean leukocyte complexity for leukocyte lineages shown (right) in tumor tissue resected from healthy pancreata (H; n = 6), pancreata tissue adjacent to PDAC (Adj; n = 13), tissue resected from PDAC margins (n = 4) or tumor centers (Ctr; n = 23), and PDACs (n = 3) from patients treated with standard-of-care CTX (+CTX). Data reflecting individual populations is provided in Supplementary Fig. S1D. D, mRNA expression of FcγR isoforms from humans with healthy pancreas versus patients with PDAC tumors. Data were compiled from gene expression available in Oncomine (https://www.oncomine.org). P values and fold change in gene expression are shown. E, FACS histogram showing relative frequency of CD64 (FcγR1) and CD16 (FcγRIII) expression on leukocytes infiltrating human PDAC. Data shown are reflective of 20 human PDACs. Lineage markers for cell types shown are as indicated in C and reflect basophils (baso), mast cells, macrophages (macs), DCs, inflammatory monocytes (iMCs), eosinophils (eos), monocytes (monos), and neutrophils (neut). Adjacent (Adj) normal indicates tissue isolated from pancreas tissue proximal to resected PDAC tumors. Andrew J. Gunderson et al. Cancer Discov 2016;6:270-285 ©2016 by American Association for Cancer Research