Systemic Monocyte and T-Cell Activation in a Patient With Human Parvovirus B19 Infection  Annette D. Wagner, M.D., Jorg J. Goronzy, M.D.,Ph.D., Eric L. Matteson, M.D., Cornelia M. Weyand, M.D.,Ph.D.  Mayo Clinic Proceedings  Volume 70, Issue 3, Pages 261-265 (March 1995) DOI: 10.4065/70.3.261 Copyright © 1995 Mayo Foundation for Medical Education and Research Terms and Conditions

Fig. 1 Constitutive expression of interleukin (IL) 1β-specific messenger RNA during parvovirus infection. Peripheral blood mononuclear cells (PBMC) from patientwith parvovirus infection during acute phase of disease (A) and 2 months later (B) were harvested. PBMC fromhealthy donorservedas control sample (C). RNA was isolated, and complementary DNA (cDNA) synthesis wasperformed. Serialdilutions ofcDNApreparations wereusedin polymerase chain reaction for semiquantification of IL 1β-specific sequences (248 base pairs [bp]), Amplification with β-actin-specific oligonucleotides served as internal control (546 bp). Results are shown as ethidium bromide stain after gel electrophoresis. During acute infection, patienthad increased concentrations of IL 1β-specific sequences. Mayo Clinic Proceedings 1995 70, 261-265DOI: (10.4065/70.3.261) Copyright © 1995 Mayo Foundation for Medical Education and Research Terms and Conditions