Suaad Alshehhi, Nicola A. McCallum, Penelope R. Haddrill

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Presentation transcript:

Quantification of RNA degradation of blood-specific markers to indicate the age of bloodstains Suaad Alshehhi, Nicola A. McCallum, Penelope R. Haddrill Forensic Science International: Genetics Supplement Series Volume 6, Pages e453-e455 (December 2017) DOI: 10.1016/j.fsigss.2017.09.175 Copyright © 2017 Elsevier B.V. Terms and Conditions

Fig. 1 ΔCq (Cqtimex−Cqtime0) for HBA, HBB, PBGD, ACTB, 18S, miR16, miR451, and U6 in total RNA extracted from blood samples stored at room temperature for 270 days. Data represents the mean of n=10. Error bars were omitted for clarity. Forensic Science International: Genetics Supplement Series 2017 6, e453-e455DOI: (10.1016/j.fsigss.2017.09.175) Copyright © 2017 Elsevier B.V. Terms and Conditions

Fig. 2 Regression analysis of the relationship between bloodstain age (up to 270 days) and RER. A second-order polynomial curve was applied to RERs: (A) HBA/HBB and (B) miR16/U6. Data represents mean of n=10. Forensic Science International: Genetics Supplement Series 2017 6, e453-e455DOI: (10.1016/j.fsigss.2017.09.175) Copyright © 2017 Elsevier B.V. Terms and Conditions