Establishment of a matrix-assisted laser desorption ionization time-of-flight mass spectrometry database for rapid identification of infectious achlorophyllous.

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Establishment of a matrix-assisted laser desorption ionization time-of-flight mass spectrometry database for rapid identification of infectious achlorophyllous green micro- algae of the genus Prototheca J. Murugaiyan, J. Ahrholdt, V. Kowbel, U. Roesler Clinical Microbiology and Infection Volume 18, Issue 5, Pages 461-467 (May 2012) DOI: 10.1111/j.1469-0691.2011.03593.x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Representative spectra (smoothed and baseline-subtracted) from different species of Prototheca and Chlorella: (a) P. stagnora, (b) P. ulmea, (c) P. wickerharmii, (d) P. zopfii genotype 1, (e) P. zopfii genotype 2, (f) P. blaschkeae, (g) P. cutis, (h) C. protothecoides and (i) C. ellipsoidea. Clinical Microbiology and Infection 2012 18, 461-467DOI: (10.1111/j.1469-0691.2011.03593.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 (a) Evaluation of uniqueness among the spectral sets of 22 Prototheca strains. The composite correlation index (CCI) matrix was calculated with MALDI Biotyper 2.0 software with the default settings: the lower bound is 3000, the upper bound is 15 000, the resolution of the mass range is four, and the number of intervals for CCI is four. (b) CCI matrix value for the strains of Prototheca included in this study (this chart has to be added as a figure using picture edit software). A CCI value near 1.0 indicates relatedness between the spectral sets, and 0 indicates no match. Clinical Microbiology and Infection 2012 18, 461-467DOI: (10.1111/j.1469-0691.2011.03593.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Score-oriented dendrogram of matrix-assisted laser desorption ionization time-of-flight mass spectrometry profiles generated by the default setting in MALDI Biotyper 2.0 (the distance measure was set at correlation, and linkage was set at complete). Clinical Microbiology and Infection 2012 18, 461-467DOI: (10.1111/j.1469-0691.2011.03593.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions