Deletion of putative sugar permease KP1_RS12820 leads to an in vivo fitness defect. Deletion of putative sugar permease KP1_RS12820 leads to an in vivo.

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Deletion of putative sugar permease KP1_RS12820 leads to an in vivo fitness defect. Deletion of putative sugar permease KP1_RS12820 leads to an in vivo fitness defect. C57BL/6 mice were inoculated intrapharyngeally with a 1:1 ratio of NTUH-K2044 (WT) and ΔKP1_RS12820 bacteria (1 × 104 CFU/mouse total) or with the WT bacteria carrying the pBBR1MCS-5 plasmid in combination with ΔKP1_RS12820 bacteria carrying pBBR1MCS-5 or pBBR1MCS-5 with the ΔKP1_RS12820 gene. A competitive index was calculated based on the ratios of WT and mutant input CFU and output CFU. The mean values (indicated by the short black lines) of the log-transformed competitive indices (10 to 17 mice in each group; two or three experiments) were compared to a hypothetical value of zero using a one-sample t test for each group. NS, not statistically significant. Rebekah M. Martin et al. mSystems 2018; doi:10.1128/mSystems.00015-18