Protein profiling of human endometrial tissues in the midsecretory and proliferative phases of the menstrual cycle Tanu Parmar, M.S., Sushama Gadkar-Sable,

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Presentation transcript:

Protein profiling of human endometrial tissues in the midsecretory and proliferative phases of the menstrual cycle Tanu Parmar, M.S., Sushama Gadkar-Sable, M.S., Lalita Savardekar, M.B.B.S., Rajendra Katkam, Ph.D., Shalmali Dharma, Ph.D., Pervin Meherji, M.D., Chander Parkash Puri, Ph.D., Geetanjali Sachdeva, Ph.D. Fertility and Sterility Volume 92, Issue 3, Pages 1091-1103 (September 2009) DOI: 10.1016/j.fertnstert.2008.07.1734 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Two-dimensional maps of endometrial proteins from (A, C) proliferative phase and (B, D) midsecretory phase, resolved either in nonlinear pH range of 3.0–11.0 (A, B), or in linear range of 4.0–7.0 (C, D) by isoelectric focusing in the first dimension and 10% SDS-PAGE in the second dimension. Arrows indicate some of the polypeptide spots displaying differential expression between two phases. Fertility and Sterility 2009 92, 1091-1103DOI: (10.1016/j.fertnstert.2008.07.1734) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (A) Some polypeptide spots (circled) displaying higher representation either in the two-dimensional map of (a) proliferative phase or (b) midsecretory phase endometria were excised from the gels and subjected to MALDI-TOF-TOF analysis. (B) Isoforms of AAT in (a) proliferative and (b) midsecretory phase endometrium. Arrow indicates the location of polypeptide spot VG excised from 2D protein gels. Fertility and Sterility 2009 92, 1091-1103DOI: (10.1016/j.fertnstert.2008.07.1734) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Immunohistochemical localization of calreticulin (a, b, c) and α1-antitrypsin precursor AAT (d, e, f) in proliferative (a, d) and midsecretory (b, e) phases. Respective negative controls are shown in c and f. (Magnification: ×40) Fertility and Sterility 2009 92, 1091-1103DOI: (10.1016/j.fertnstert.2008.07.1734) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Semiquantitative comparison of (A) calreticulin and (B) α1-antitrypsin immunostaining in glandular and stromal compartments of proliferative and midsecretory phase endometria. ∗Indicates statistical significance of the difference between two groups. Fertility and Sterility 2009 92, 1091-1103DOI: (10.1016/j.fertnstert.2008.07.1734) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 5 Flow cytometric analysis demonstrating change in the percentage of Ishikawa cells expressing calreticulin or α1-antitrypsin precursor after stimulation with estrogen or progesterone. Fertility and Sterility 2009 92, 1091-1103DOI: (10.1016/j.fertnstert.2008.07.1734) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 6 Comparison of two-dimensional protein profiles of (A) endometrial tissue and (B) uterine fluid during the midsecretory phase of the cycle. Arrows indicate the matched polypeptide spots. VG: α1-antitrypsin precursor, GT4: heat-shock protein 27, and VS: transferrin. Fertility and Sterility 2009 92, 1091-1103DOI: (10.1016/j.fertnstert.2008.07.1734) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions