Parameters affecting successful transplantation of frozen-thawed human fetal ovaries into immunodeficient mice  Ronit Abir, PhD, Raoul Orvieto, MD, MSc, Hila Raanani, MD, Dov Feldberg, MD, Shmuel Nitke, MD, Benjamin Fisch, MD, PhD  Fertility and Sterility  Volume 80, Issue 2, Pages 421-428 (August 2003) DOI: 10.1016/S0015-0282(03)00658-7

FIGURE 1 A propandiol frozen-thawed ovary from a normal fetus 21 gestational weeks of age (control). The arrow indicates primordial follicles. Hematoxylin–eosin, ×400. Abir. Human fetal ovaries in immunodeficient mice.Fertil Steril 2003. Fertility and Sterility 2003 80, 421-428DOI: (10.1016/S0015-0282(03)00658-7)

FIGURE 2 A primary follicle (P) identified 4 months after grafting. The ovaries were frozen-thawed with propandiol and originated from a 47,XXX fetus 21 gestational weeks of age. Note the brown proliferating-cell nuclear antigen staining in most of the granulosa cells and in the nuclei of the oocyte. Magnification, ×400. Abir. Human fetal ovaries in immunodeficient mice.Fertil Steril 2003. Fertility and Sterility 2003 80, 421-428DOI: (10.1016/S0015-0282(03)00658-7)

FIGURE 3 A human fetal ovary 6.5 months after renal grafting. The ovaries were frozen-thawed in dimethyl sulfoxide and originated from a 21-gestational-week-old fetus with trisomy 21. The arrow indicates secondary follicles. Note the brown proliferating-cell nuclear antigen staining in granulosa cells and oocytes. Magnification, ×200. Abir. Human fetal ovaries in immunodeficient mice.Fertil Steril 2003. Fertility and Sterility 2003 80, 421-428DOI: (10.1016/S0015-0282(03)00658-7)

FIGURE 4 An ovary from a fetus after 6 months of subcutaneous grafting. The ovaries were frozen-thawed in dimethyl sulfoxide and originated from a normal fetus 21 gestational weeks of age. The arrow indicates the initial formation of primordial follicles. Hematoxylin–eosin, ×400. Abir. Human fetal ovaries in immunodeficient mice.Fertil Steril 2003. Fertility and Sterility 2003 80, 421-428DOI: (10.1016/S0015-0282(03)00658-7)

FIGURE 5 A negative control of the same graft shown in Figure 3. Note the secondary follicle, the overall purple-blue staining, and the lack of brown proliferating-cell nuclear antigen staining. Magnification, ×400. Abir. Human fetal ovaries in immunodeficient mice.Fertil Steril 2003. Fertility and Sterility 2003 80, 421-428DOI: (10.1016/S0015-0282(03)00658-7)