Effects of the FSH receptor gene polymorphism p

Slides:

Advertisements

Similar presentations

GnRH agonist and GnRH antagonist protocols in ovarian stimulation: differential regulation pathway of aromatase expression in human granulosa cells Mohamad.

Advertisements

In-vitro culture system for mesenchymal progenitor cells derived from waste human ovarian follicular fluid Federica Riva, Claudia Omes, Roberto Bassani,

Kathryn J. Woad, Deborah Prendergast, Ingrid M. Winship, Andrew N

Kam-Hei So, Cheuk-Lun Lee, William S.B. Yeung, Kai-Fai Lee

Polymorphisms of nucleotide-excision repair genes may contribute to sperm DNA fragmentation and male infertility Aihua Gu, Guixiang Ji, Yong Zhou, Yan.

Polymorphism of CAG and GGN repeats of androgen receptor gene in women with polycystic ovary syndrome Chun Yuan, Chao Gao, Yi Qian, Ying Liu, Shi-Wen.

Live births after management of severe OHSS by GnRH antagonist administration in the luteal phase TG Lainas, IA Sfontouris, IZ Zorzovilis, GK Petsas,

Progesterone stimulates expression of follistatin splice variants Fst288 and Fst315 in the mouse uterus R.G. Craythorn, W.R. Winnall, F. Lederman, E.J.

Low-dosage clomiphene reduces premature ovulation rates and increases transfer rates in natural-cycle IVF M von Wolff, M Nitzschke, P Stute, N Bitterlich,

Brain-derived neurotrophic factor expression in granulosa lutein cells

Luteinizing hormone/chorionic gonadotrophin receptor overexpressed in granulosa cells from polycystic ovary syndrome ovaries is functionally active Venkateswarlu.

Corifollitropin alfa doses based on body weight: clinical overview of drug exposure and ovarian response William L. Ledger, Bart C.J.M. Fauser, Paul.

Mid-luteal progesterone concentrations are associated with live birth rates during ovulation induction J.-C. Arce, A. Balen, P. Platteau, G. Pettersson,

L. L. Sun, Z. Y. Sun, P. Zhang, X. W. Zhai, J. Tang, Q. J. Pan, Q. H

Huan Yang, Caixia Lei, Wei Zhang Reproductive BioMedicine Online

Polymorphisms in gonadotropin and gonadotropin receptor genes as markers of ovarian reserve and response in in vitro fertilization Antonio La Marca,

Pharmacokinetics and follicular dynamics of corifollitropin alfa versus recombinant FSH during ovarian stimulation for IVF Bart C.J.M. Fauser, Michael.

Preovulatory progesterone concentration associates significantly to follicle number and LH concentration but not to pregnancy rate Claus Yding Andersen,

Withdrawal of GnRH agonist decreases oestradiol and VEGF concentrations in high responders Li-Jun Ding, Bin Wang, Xiao-Yue Shen, Gui-Jun Yan, Ning-Yuan.

Helena T. A. van Tol, Johannes C. M

Folate-metabolizing gene variants and pregnancy outcome of IVF

Conventional morphology performs better than morphokinetics for prediction of live birth after day 2 transfer Aisling Ahlstrom, Hannah Park, Christina.

No association between endogenous LH and pregnancy in a GnRH antagonist protocol: part II, recombinant FSH George Griesinger, Daniel B. Shapiro, Efstratios.

Alterations in mouse embryo intracellular pH by DMO during culture impair implantation and fetal growth Deirdre L. Zander-Fox, Megan Mitchell, Jeremy.

MiR-142-3p is a novel regulator of cell viability and proinflammatory signalling in endometrial stroma cells Christin Susanna Kästingschäfer, Sebastian.

L. L. van Loendersloot, M. van Wely, S. Repping, F. van der Veen, P. M

High serum oestradiol concentrations in IVF cycles increase the risk of pregnancy complications related to abnormal placentation Jacob Farhi, Avi Ben.

Publication productivity in IVF in Europe, 1990–2006

Double stimulations during the follicular and luteal phases of poor responders in IVF/ICSI programmes (Shanghai protocol) Yanping Kuang, Qiuju Chen,

Silke Klocke, Claudia Tappehorn, Georg Griesinger

Short follicular phase of stimulation following corifollitropin alfa or daily recombinant FSH treatment does not compromise clinical outcome: a retrospective.

Impact of high serum progesterone during the late follicular phase on IVF outcome Charlotte Sonigo, Géraldine Dray, Clémence Roche, Isabelle Cédrin-Durnerin,

Why are reproductive cancers more common in nulliparous women?

Invited commentary: A single nucleotide polymorphism in BMP15 is associated with high response to controlled ovarian hyperstimulation Ronit Abir, Benjamin.

Fabian Lunger, Anni P. Vehmas, Barbara G

Does supplementation of in-vitro culture medium with melatonin improve IVF outcome in PCOS? Mi Kyoung Kim, Eun A. Park, Hyung Joon Kim, Won Yun Choi,

Utility of age-specific serum anti-Müllerian hormone concentrations

In-vitro culture system for mesenchymal progenitor cells derived from waste human ovarian follicular fluid Federica Riva, Claudia Omes, Roberto Bassani,

Maternal concentrations of human chorionic gonadotrophin in very early IVF pregnancies and duration of pregnancy: a follow-up study Tom G. Tanbo, Manuela.

Effect of N-acetyl-cysteine after ovarian drilling in clomiphene citrate-resistant PCOS women: a pilot study A. Nasr Reproductive BioMedicine Online

Single-nucleotide polymorphisms in the promoter region influence the expression of the human follicle-stimulating hormone receptor Alain Wunsch, M.Sc.,

Ovarian reserve determinations suggest new function of FMR1 (fragile X gene) in regulating ovarian ageing Norbert Gleicher, Andrea Weghofer, David H.

M. Dieterich, M. Bolz, T. Reimer, S. Costagliola, B. Gerber

Cardiovascular autonomic dysfunction in women with polycystic ovary syndrome: a systematic review and meta-analysis Juan Gui, Rui-hao Wang Reproductive.

Sequential use of letrozole and gonadotrophin in women with poor ovarian reserve: a randomized controlled trial Vivian Chi Yan Lee, Carina Chi Wai Chan,

Decreased expression of FOXA2 promotes eutopic endometrial cell proliferation and migration in patients with endometriosis Anping Lin, Juan Yin, Chao.

The incidence of long heterochromatic polymorphism variants in infants conceived through assisted reproductive technologies Andrew Wilson, Katherine.

Follicular flushing in natural cycle IVF does not affect the luteal phase – a prospective controlled study M. von Wolff, A. Kohl Schwartz, P. Stute,

The relationship between sperm head retardance using polarized light microscopy and clinical outcomes Belinda G. Vermey, Michael G. Chapman, Simon Cooke,

Mid-luteal serum progesterone concentrations govern implantation rates for cryopreserved embryo transfers conducted under hormone replacement John L.

V Isachenko, E Isachenko, R Kreienberg, M Woriedh, JM Weiss

Michael J. Levy, William Ledger, Efstratios M. Kolibianakis, Pieta C

Swapna S. Desai, Swati K. Achrekar, Smita R. Paranjape, Sadhana K

Intra-cycle variation of the uterine cavity indentation assessed with three-dimensional ultrasound in natural and stimulated cycles Sotirios H. Saravelos,

LH concentrations do not correlate with pregnancy in rFSH/GnRH antagonist cycles Kevin Doody, Paul Devroey, Keith Gordon, Han Witjes, Bernadette Mannaerts

Is polycystic ovary syndrome a sexual conflict? A review

Sperm DNA fragmentation index as a promising predictive tool for male infertility diagnosis and treatment management – meta-analyses Daniele Santi, Giorgia.

Stephen C. Collins, Esther Chan Reproductive BioMedicine Online

Anna Pia Ferraretti, Paul Devroey, M. Cristina Magli, Luca Gianaroli

Does exogenous LH in ovarian stimulation improve assisted reproduction success? An appraisal of the literature Micah J. Hill, Gary Levy, Eric D. Levens

Genome-wide analysis shows no genomic predictors of ovarian response to stimulation by exogenous FSH for IVF Jeroen van Disseldorp, Lude Franke, Rene.

Aryl hydrocarbon receptor antagonists attenuate the deleterious effects of benzo[a]pyrene on isolated rat follicle development Michael S. Neal, Anne.

FMR1 and AKT/mTOR signalling pathways: potential functional interactions controlling folliculogenesis in human granulosa cells Julia Rehnitz, Diego D.

Anti-Müllerian hormone is highly expressed and secreted from cumulus granulosa cells of stimulated preovulatory immature and atretic oocytes Alon Kedem-Dickman,

Factors related to successful ovulation induction in patients with WHO group II anovulatory infertility C.M. Howles, V. Alam, D. Tredway, R. Homburg,

Norbert Gleicher, Andrea Weghofer, David H. Barad

Human granulosa-lutein cell in vitro production of progesterone, inhibin A, inhibin B, and activin A are dependent on follicular size and not the presence.

James D. Stanger, Long Vo, John L. Yovich, Ghanim Almahbobi

Follicle-stimulating hormone receptor polymorphisms in women with normogonadotropic anovulatory infertility Joop S.E Laven, Ph.D., Annemarie G.M.G.J.

High oestradiol concentration after ovarian stimulation is associated with lower maternal serum beta-HCG concentration and neonatal birth weight Suying.

Presentation transcript:

Effects of the FSH receptor gene polymorphism p Effects of the FSH receptor gene polymorphism p.N680S on cAMP and steroid production in cultured primary human granulosa cells Verena Nordhoff, Barbara Sonntag, Dominik von Tils, Martin Götte, Andreas N. Schüring, Jörg Gromoll, Klaus Redmann, Livio Casarini, Manuela Simoni Reproductive BioMedicine Online Volume 23, Issue 2, Pages 196-203 (August 2011) DOI: 10.1016/j.rbmo.2011.04.009 Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Basal and stimulated oestradiol (A) and progesterone (B) production of day-2 GC cultures with the NN and SS genotypes. Applying two-way-ANOVA, oestradiol, but not progesterone, concentration was significantly different with respect to FSHR variant (oestradiol, P=0.045) and irrespective of stimulatory treatment. Asterisk=significant differences between individual groups; NS=not statistically significant. Reproductive BioMedicine Online 2011 23, 196-203DOI: (10.1016/j.rbmo.2011.04.009) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Representative time course of FSH receptor gene (A–C), inhibin (D–F), oestradiol receptor α (G–I) and oestradiol receptor β (J–L) expression during prolonged culture of human granulosa cells of a heterozygous (NS) patient. GC were cultured for the indicated time points and processed for immunofluorescence microscopy. Green=investigated antigens, red=phalloidin counterstaining of the actin cytoskeleton. (M) Time-dependent re-appearance of FSHR in granulosa cells of a heterozygous (NS) patient measured by flow cytometry. Bar=50μm for panels A–L. Reproductive BioMedicine Online 2011 23, 196-203DOI: (10.1016/j.rbmo.2011.04.009) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Comparison of mean dose response curves following FSH stimulation between (A) NN and SS cAMP, (C) NN and SS progesterone and (E) NN and SS oestradiol following a non-linear fit in normalized semi-logarithmic distributions. Bars=standard deviations. Representation of (B) NN and SS cAMP ED50, (D) NN and SS progesterone ED50 and (F) NN and SS oestradiol ED50 with box and whisker plots. Data sets with abnormal response trends or inaccurate ED values were excluded from the analysis, no significant difference with Mann–Whitney U-test analysis was observed. Reproductive BioMedicine Online 2011 23, 196-203DOI: (10.1016/j.rbmo.2011.04.009) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions