Recombinant birch pollen allergens (rBet v 1 and rBet v 2) contain most of the IgE epitopes present in birch, alder, hornbeam, hazel, and oak pollen:

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Presentation transcript:

Recombinant birch pollen allergens (rBet v 1 and rBet v 2) contain most of the IgE epitopes present in birch, alder, hornbeam, hazel, and oak pollen: A quantitative IgE inhibition study with sera from different populations Verena Niederberger, MDa, Gabrielle Pauli, MDb, Hans Grönlundc, Renate Fröschla, Helmut Rumpold, MDa, Dietrich Kraft, MDd, Rudolf Valenta, MDd, Susanne Spitzauer, MDa Journal of Allergy and Clinical Immunology Volume 102, Issue 4, Pages 579-591 (October 1998) DOI: 10.1016/S0091-6749(98)70273-8 Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 1 Inhibition of IgE binding to nitrocellulose-blotted natural tree pollen extracts (A, Betula verrucosa/birch; B, Alnus glutinosa/alder; and C, Carpinus betulus/hornbeam) with a combination of rBet v 1 and rBet v 2. Nitrocellulose-blotted tree pollen extracts (D, Corylus avellana/hazel; and E, Quercus alba/oak) were probed with sera from 11 representative patients allergic to tree pollen (panels 2 to 12) and, for control purposes, with serum from a nonallergic individual (panel 1). Sera were preabsorbed with a mixture of rBet v 1 and rBet v 2 (lanes +) or, for control purposes, with BSA (lanes-). Molecular weights are displayed at left. Black arrows indicate position of Bet v 1 and Bet v 1-homologous allergens, and grey arrow indicate those of Bet v 2 and Bet v 2-homologous allergens. Journal of Allergy and Clinical Immunology 1998 102, 579-591DOI: (10.1016/S0091-6749(98)70273-8) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 2 Photodocumentation of immediate-type skin reactions to recombinant birch pollen allergens and natural tree pollen extracts of 5 representative patients (al, a2, a3, a4, a5; see Table III). Application order of test substances is indicated in insert. Journal of Allergy and Clinical Immunology 1998 102, 579-591DOI: (10.1016/S0091-6749(98)70273-8) Copyright © 1998 Mosby, Inc. Terms and Conditions