Hyaline cartilage cells outperform mandibular condylar cartilage cells in a TMJ fibrocartilage tissue engineering application L. Wang, M.S., M. Lazebnik,

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Hyaline cartilage cells outperform mandibular condylar cartilage cells in a TMJ fibrocartilage tissue engineering application L. Wang, M.S., M. Lazebnik, M.S. Detamore, Ph.D. Osteoarthritis and Cartilage Volume 17, Issue 3, Pages 346-353 (March 2009) DOI: 10.1016/j.joca.2008.07.004 Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Cell number per construct at weeks 0 and 6 (n=4). Gluco denotes glucosamine. Symbol * means statistically significant difference between ankle groups and TMJ groups. Error bars represent standard deviations. Osteoarthritis and Cartilage 2009 17, 346-353DOI: (10.1016/j.joca.2008.07.004) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 GAG content per construct at weeks 0 and 6 (n=4). Gluco denotes glucosamine. Symbol * means statistically significant difference between ankle groups and TMJ groups. Symbol # means statistically significant difference compared to the respective control group. Error bars represent standard deviations. Osteoarthritis and Cartilage 2009 17, 346-353DOI: (10.1016/j.joca.2008.07.004) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Hydroxyproline content per construct at weeks 0 and 6 (n=4) (multiply by a factor of 11.5 for collagen content). Gluco denotes glucosamine. Symbol * means statistically significant difference between ankle groups and TMJ groups. Symbol # means statistically significant difference compared to the respective control group. Error bars represent standard deviations. Osteoarthritis and Cartilage 2009 17, 346-353DOI: (10.1016/j.joca.2008.07.004) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 IHC test for types I and II collagen at week 6 (n=2). Positive staining is purple in color. The scale bar is 100μm. CI=type I collagen and CII=type II collagen. Osteoarthritis and Cartilage 2009 17, 346-353DOI: (10.1016/j.joca.2008.07.004) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Double IHC staining for types I and II collagen in sagittal sections from native tissues. Positive staining of type I collagen is brown in color. Positive staining of type II collagen is purple in color. Note that the hyaline cartilage is stained almost exclusively for type II collagen, whereas in sharp contrast the mandibular condylar cartilage consists of distinct zones dominated either by collagen I (superficial zone) or collagen II (mature and hypertrophic zones) staining. The scale bar is 100μm. Osteoarthritis and Cartilage 2009 17, 346-353DOI: (10.1016/j.joca.2008.07.004) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 IHC staining for aggrecan at week 6 (n=2). Arrows indicate examples of positive staining of aggrecan (light purple color). The black color indicates PGA debris. The scale bar is 400μm. Osteoarthritis and Cartilage 2009 17, 346-353DOI: (10.1016/j.joca.2008.07.004) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions