Molecular diagnostic and genetic characterization of highly pathogenic viruses: application during Crimean–Congo haemorrhagic fever virus outbreaks in.

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

Molecular characterization of dengue virus 1 from autochthonous dengue fever cases in Croatia I.C. Kurolt, L. Betica-Radić, O. Daković-Rode, L. Franco,

Sequence and structure relatedness of matrix protein of human respiratory syncytial virus with matrix proteins of other negative-sense RNA viruses K.

P.-Y. Lévy Clinical Microbiology and Infection

Molecular evolution of Mycobacterium tuberculosis

Human papilloma viruses and cancer in the post-vaccine era

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated over a 2-year period in a Qatari hospital from multinational patients

M. Cogliati, R. D'Amicis, A.M. Tortorano

Herpes zoster in non-hospitalized children

H. Un, N. Johnson, A. Vos, T. Muller, A.R. Fooks, O. Aylan

A.K. Reddy, P. Garg, I. Kaur Clinical Microbiology and Infection

J.-P. Van geertruyden Clinical Microbiology and Infection

Genetic detection of Dobrava/Belgrade virus in a Czech patient with Haemorrhagic fever with renal syndrome A. Papa, H. Zelená, D. Barnetová, L. Petroušová

Virological tools to diagnose and monitor hepatitis C virus infection

Refugee crisis and re-emergence of forgotten infections in Europe

R. Cantón Clinical Microbiology and Infection

W. Li, L. Liu, X. Jiang, X. Guo, M. Garnier, D. Raoult, P. Parola

Non-antibiotic strategies for sepsis

Detection of Anaplasma phagocytophilum in wild boar in Slovenia

The importance of enterovirus surveillance in a Post-polio world

Evaluation of viral co-infections in hospitalized and non-hospitalized children with respiratory infections using microarrays S. Kouni, P. Karakitsos,

Prevalence of Toscana virus antibodies in residents of Croatia

S. Richard, P. Seng, P. Parola, D. Raoult, B. Davoust, P. Brouqui

Vector control: a cornerstone in the malaria elimination campaign

Viruses detected by systematic multiplex polymerase chain reaction in adults with suspected community-acquired pneumonia attending emergency departments.

Tracking the naturally occurring mutations across the full-length genome of hepatitis B virus of genotype D in different phases of chronic e-antigen-negative.

P. Seng, J.M. Rolain, D. Raoult, P. Brouqui

Male patient with acute hepatitis E in Genoa, Italy: figatelli (pork liver sausage) as probable source of the infection A.R. Garbuglia, A.I. Alessandrini,

Next-generation sequencing technology in clinical virology

Hepatitis C virus and interferon type III (interferon-λ3/interleukin-28B and interferon-λ4): genetic basis of susceptibility to infection and response.

F. Magurano Clinical Microbiology and Infection

Screening for Middle East respiratory syndrome coronavirus infection in hospital patients and their healthcare worker and family contacts: a prospective.

Early invasive fungal infections and colonization in patients with cirrhosis admitted to the intensive care unit E. Theocharidou, B. Agarwal, G. Jeffrey,

Human infection with H9N2 avian influenza in northern China

H. Yongfeng, Y. Fan, D. Jie, Y. Jian, Z. Ting, S. Lilian, Q. Jin

Elements of design: the knowledge on which we build

Enterovirus D68 nosocomial outbreak in elderly people, France, 2014

Multiple genotypes and subtypes of hepatitis B and C viruses in Belarus: similarities with Russia and western European influences C.M. Olinger, N.V.

A novel phlebovirus in Albanian sandflies

Reena Ghildyal, Geoff Hogg, John Mills, Jayesh Meanger

G. Pappas Clinical Microbiology and Infection

A. Papa, K. Xanthopoulou, S. Gewehr, S. Mourelatos

P. Sidira, H.C. Maltezou, A.-B. Haidich, A. Papa

Metagenomics and probiotics

Detection of Wolbachia genes in a patient with non-Hodgkin's lymphoma

Whole genome-based phylogenetic analysis of Rickettsiae

Sequence and structure relatedness of matrix protein of human respiratory syncytial virus with matrix proteins of other negative-sense RNA viruses K.

The practice of travel medicine in Europe

Systematic review of antibiotic consumption in acute care hospitals

Molecular characterization of dengue virus 1 from autochthonous dengue fever cases in Croatia I.C. Kurolt, L. Betica-Radić, O. Daković-Rode, L. Franco,

MW polyomavirus and STL polyomavirus present in tonsillar tissues from children with chronic tonsillar disease J. Peng, K. Li, C. Zhang, Q. Jin Clinical.

Pandemic lineages of extraintestinal pathogenic Escherichia coli

Characterization of clinical strains of Aspergillus terreus complex: molecular identification and antifungal susceptibility to azoles and amphotericin.

Emergence of Crimean–Congo haemorrhagic fever in Greece

R. Slinger, I. Moldovan, N. Barrowman, F. Chan

Molecular evidence of Anaplasma phagocytophilum infection in wild cervids and feeding Ixodes ricinus ticks from west-central Poland J. Michalik, J. Stańczak,

H. Leblebicioglu, C. Eroglu Clinical Microbiology and Infection

C. Alkan, A. B. Allal-Ikhlef, S. Alwassouf, A. Baklouti, G

Clinical Microbiology and Infection

Molecular epidemiology and genetic diversity of human astrovirus in South Korea from 2002 to 2007 A.Y. Jeong, H.S. Jeong, M.Y. Jo, S.Y. Jung, M.S. Lee,

K. Kaier, N.T. Mutters, U. Frank Clinical Microbiology and Infection

Detection of a new subgenotype of hepatitis B virus genotype A in Cameroon but not in neighbouring Nigeria J.M. Hübschen, P.O. Mbah, J.C. Forbi, J.A.

The atypical pneumonias: clinical diagnosis and importance

Facilitating learning and change in physicians: Implications for a system of continuing medical education in Europe Robert D. Fox Clinical Microbiology.

Influence of climate change on the incidence and impact of arenavirus diseases: a speculative assessment J.C. Clegg Clinical Microbiology and Infection

M. Biçmen, Z. Gülay, S.V. Ramaswamy, D.M. Musher, D. Gür

G.C. Schito Clinical Microbiology and Infection

Sandfly fever virus outbreak in Cyprus

J. -H. Lin, S. -C. Chiu, J. -C. Cheng, H. -W. Chang, K. -L. Hsiao, Y

The future of diagnostic bacteriology

Presentation transcript:

Molecular diagnostic and genetic characterization of highly pathogenic viruses: application during Crimean–Congo haemorrhagic fever virus outbreaks in Eastern Europe and the Middle East C. Filippone, P. Marianneau, S. Murri, N. Mollard, T. Avsic-Zupanc, S. Chinikar, P. Desprès, V. Caro, A. Gessain, N. Berthet, N. Tordo Clinical Microbiology and Infection Volume 19, Issue 2, Pages E118-E128 (February 2013) DOI: 10.1111/1469-0691.12075 Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1. Flow chart of the experimental procedure based on resequencing microarrray for the detection of highly pathogenic viruses. Clinical Microbiology and Infection 2013 19, E118-E128DOI: (10.1111/1469-0691.12075) Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2. Phylogenetic Tree. Phylogenetic analysis of Crimean–Congo haemorrhagic fever virus (CCHFV) variants was performed using a 531-bp sequence in the CCHFV L segment encoding RNA-dependent RNA polymerase (position 2717–3248) and the neighbour-joining method with BioNumerics software for Windows (version 5.1, Applied Maths). Sequences were: (i) retrieved from GenBank (L); (ii) experimentally obtained from supernatants of CCHFV-infected cell cultures or from infected human serum (Seq); or (iii) the results output from the microarray (Chip) (*). Clinical Microbiology and Infection 2013 19, E118-E128DOI: (10.1111/1469-0691.12075) Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions