This Month in Gastroenterology Laura Harrell, Eugene B. Chang  Gastroenterology  Volume 129, Issue 1, Pages 1-3 (July 2005) DOI: 10.1053/j.gastro.2005.05.044 Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 1 Identification of HNP1-3. (A) Normalized ProteinChip arrays profiles of the immunodepletion assays of microdissected colonic tissue. For HNP1-3 identification, colon cancer tissue was used as starting material for immunodepletion assays by the corresponding monoclonal antibody. The negative control was performed without antibodies. The peaks at 3.37, 3.44, and 3.49 kilodaltons representing HNP1-3 were detectable in a higher amount in the negative control. (B) Normalized ProteinChip arrays spectra of the immunocapturing assay of microdissected colon carcinoma tissue. HNP1-3 were captured from microdissected colon carcinoma tissue by the corresponding monoclonal antibody bound on IDM beads. The peaks at 3.37, 3.44, and 3.48 kilodaltons representing HNP1-3 were clearly detectable in samples eluated from the IDM beads. In control assays without the specific antibody, no HNP1-3 were captured. (C) Areas with positive and negative reaction in IHC were microdissected and analyzed on ProteinChip arrays. Signals with a molecular mass of 3.37, 3.44, and 3.49 kilodaltons representing HNP1-3 were detectable in protein lysates from positive areas and were absent in the negative areas. Gastroenterology 2005 129, 1-3DOI: (10.1053/j.gastro.2005.05.044) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 2 Immunohistochemistry (IHC) of HNP1-3 visualized by normal and laser scanning microscopy (LSM; positive reaction is depicted in blue). (A and B) IHC of HNP1-3 on colon carcinoma. (A) Increased signal intensity in carcinoma (Ca) structures (overview with 25× magnification; N, normal epithelium). (B) Strong positive reaction of invasive growing carcinoma complexes (Ca; magnification of 120×). Gastroenterology 2005 129, 1-3DOI: (10.1053/j.gastro.2005.05.044) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 3 Overall survival of patients diagnosed with NAFLD in Olmsted County, Minnesota, between January 1, 1980 and January 1, 2000. Survival is compared with the general population of Minnesota of the same age and sex. Gastroenterology 2005 129, 1-3DOI: (10.1053/j.gastro.2005.05.044) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 4 L-glutamine potentiation of Hsp72 induction by L-glutamine is associated with decreased caspase 3 activation. Two hours after heat shock, cells were exposed to 20 μmol/L camptothecin, a topoisomerase II inhibitor. The glutamine effect on Hsp72 was associated with the delayed appearance and decreased abundance of (A) cleaved caspase-3 (17-19 kilodalton) and (B) large and small cleaved fragments of PARP. Gastroenterology 2005 129, 1-3DOI: (10.1053/j.gastro.2005.05.044) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 5 GH administration improves colon disease activity. (A) GHR expressing cells were identified in PBS treated WT and IL-10 null mice by IHC using the AL-47 GHR antibody. (A) WT, (B) IL-10 null, and (C) negative control. Representative positive CEC and LPMC are identified with the closed arrows. Gastroenterology 2005 129, 1-3DOI: (10.1053/j.gastro.2005.05.044) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 6 Bright-field photomicrographs of representative cecum sections stained with Sirius red for collagen. HSA control rats treated with vehicle or GH had low fibrosis scores (< 1.0) with the majority of collagen deposition located within the submucosa, as is found in the normal cecum (A and B). PG-APS rats showed obvious increases in transmural collagen deposition (C–F), validated by significant increases in mean histological scores for fibrosis. PG-APS rats treated with GH had a modest, but significant, reduction in histological scores for fibrosis compared with PG-APS-injected rats given vehicle, as illustrated also by the representative sections in C and E versus D and F. Gastroenterology 2005 129, 1-3DOI: (10.1053/j.gastro.2005.05.044) Copyright © 2005 American Gastroenterological Association Terms and Conditions