Putative targets of miRNAs directly induced by p53 with down-regulated mRNA- and de novo protein synthesis or reduced de novo protein synthesis only. Putative.

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binding sites 58 of the 473 unambiguously assigned phosphorylation sites are predicted by Scansite to be sites for binding. 50 of these correspond.

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Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.

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Plasma CEP adducts and autoantibodies by donor age.

Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,

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Two-dimensional electrophoresis results and validation with Western blotting. Two-dimensional electrophoresis results and validation with Western blotting.

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Significantly enriched phosphorylation motifs from up-regulated phosphopeptides by Motif-X analysis. Significantly enriched phosphorylation motifs from.

Visual validation of the computational outputs.

Epitope Mapping Performance using a single peptide microarray.

Frequency distribution of the effect size measure (ESM) of sperm protein spots of type-1 diabetic (A), type-2 diabetic (B) and non-diabetic obese (C) patients.

Novel p53 target genes identified by RNA-Seq, pSILAC and ChIP-Seq.

Assay of NOS activity. Assay of NOS activity. Box show total NOS activity, the calcium-dependent activity of the constitutive isoforms of NOS (eNOS and.

Membrane protein overexpression affects the pH of the culture and protein secretion.A, the pH of the culture of GFP fusion-overexpressing cells and the.

Bottom-up proteomic characterization of MALDI IMS samples.

Genome-wide analysis of p53 occupancy.

Representative example of LAXIC performance for complex plant phosphoproteome. Representative example of LAXIC performance for complex plant phosphoproteome.A.

Success rates in validation of antibodies from external providers

Results from the Morris water task.

Technical reproducibility and biological variability.

Identification of de novo synthesized UPF1 target proteins.

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Schematic model of effector pathways that mediate tumor suppression by p53. Schematic model of effector pathways that mediate tumor suppression by p53.

MRNAs that show increased protein synthesis following UPF1 depletion are enriched for those with very long 3′UTRs. mRNAs that show increased protein synthesis.

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Analysis of newly synthesized proteins by combined pulsed SILAC and click chemistry enrichment. Analysis of newly synthesized proteins by combined pulsed.

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Validation of p53- and miRNA-mediated down-regulation

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Interaction networks of the regulated phosphoproteins.

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Plot of the deviation of the predicted pI value of every peptide spectrum from the average pI calculated for each fraction for validated (a) and non-validated.

Distribution of the phosphoproteins based on GO analysis, including biological process (Left) and cellular component (Right). Distribution of the phosphoproteins.

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Biochemical characterization of the protein phosphatases Saci-PTP.

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Statistical evaluation of CD56+ NK cell subset data.

Changes in protein expression during distinct stages of NK cell differentiation. Changes in protein expression during distinct stages of NK cell differentiation.

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Putative targets of miRNAs directly induced by p53 with down-regulated mRNA- and de novo protein synthesis or reduced de novo protein synthesis only. Putative targets of miRNAs directly induced by p53 with down-regulated mRNA- and de novo protein synthesis or reduced de novo protein synthesis only. Predicted miRNA targets with (A) down-regulated mRNA- and de novo protein synthesis, (B) reduced de novo protein synthesis only are displayed. Known miRNA targets are underlined and targets experimentally validated in this study are marked with a black arrow. Proteins that showed a heterogeneous distribution of fold changes in independent experiments (class II candidates, see Experimental procedures) are marked with an asterisk (*). Sabine Hünten et al. Mol Cell Proteomics 2015;14:2609-2629 © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.