Systematic analysis of interacting proteins with known binding affinities Systematic analysis of interacting proteins with known binding affinities Selection.

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Systematic analysis of interacting proteins with known binding affinities Systematic analysis of interacting proteins with known binding affinities Selection of PPIs for the affinity‐based interaction reference set (AIRS) from the PDBbind (Wang et al, 2004) and the Protein–Protein‐Interaction Affinity Database 2.0 (Kastritis et al, 2011).Selected protein pairs cover a broad range of published binding affinities; PPIs with low (> 10−6 M), medium (> 10−8 M), and high binding affinities (≤ 10−8 M) were sub‐grouped in the AIRS.Recovery rates of PPIs from affinity‐based sub‐groups with BRET and LuC assays. Significance was calculated by two‐sided Fisher's exact test; *P < 0.05.Success of PPI detection from AIRS sub‐groups considering single and double LuTHy readouts.Scatter plot depicting the relationships between published binding affinities (KD) and in‐cell BRET50 measurements for 25 interactions in AIRS; BRET50 values are the mean from two independent experiments. Significance was calculated by two‐tailed Spearman correlation; ***P < 0.001. Philipp Trepte et al. Mol Syst Biol 2018;14:e8071 © as stated in the article, figure or figure legend