Olga A. Gra, Julia V. Sidorova, Eugene A. Nikitin, Alexander Y

Slides:

Advertisements

Similar presentations

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Advertisements

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis Philippe Szankasi, David.

Alexander A. Morley, Sue Latham, Michael J. Brisco, Pamela J

Mutation Screening of EXT1 and EXT2 by Denaturing High-Performance Liquid Chromatography, Direct Sequencing Analysis, Fluorescence in Situ Hybridization,

Preferential Expansion of Vγ9-JγP/Vδ2-Jδ3 γδ T Cells in Nasal T-Cell Lymphoma and Chronic Active Epstein-Barr Virus Infection Michiko K. Oyoshi, Hiroshi.

Effectiveness of Capillary Electrophoresis Using Fluorescent-Labeled Primers in Detecting T-Cell Receptor γ Gene Rearrangements Timothy C. Greiner, Ronald.

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large.

Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses Narasimhan Nagan, Nicole E. Faulkner,

Kirby Siemering, Shehnaaz S. M. Manji, Wendy M

Effectiveness of Capillary Electrophoresis Using Fluorescent-Labeled Primers in Detecting T-Cell Receptor γ Gene Rearrangements Timothy C. Greiner, Ronald.

Detection of Clonally Restricted Immunoglobulin Heavy Chain Gene Rearrangements in Normal and Lesional Skin Minakshi Nihal, Debra Mikkola, Gary S. Wood

Analysis of T-Cell Clonality Using Laser Capture Microdissection and High-Resolution Microcapillary Electrophoresis Evgeny Yakirevich, Cynthia L. Jackson,

Comparison of BIOMED-2 Versus Laboratory-Developed Polymerase Chain Reaction Assays for Detecting T-Cell Receptor-γ Gene Rearrangements Keyur P. Patel,

Pooja Chitgopeker, Debjani Sahni Journal of Investigative Dermatology

Increased Sensitivity of the Roche COBAS AMPLICOR HCV Test, Version 2

Analysis of HER2 Gene Amplification Using an Automated Fluorescence in Situ Hybridization Signal Enumeration System Rachel Stevens, Imad Almanaseer,

Cornelis J. J. Huijsmans, Jeroen Poodt, Paul H. M. Savelkoul, Mirjam H

Molecular Diagnostic Approach to Non-Hodgkin's Lymphoma

Jeung-Yeal Ahn, Katie Seo, Olga Weinberg, Scott D. Boyd, Daniel A

Rapid Real-Time PCR Assays for Detection of Klebsiella pneumoniae with the rmpA or magA Genes Associated with the Hypermucoviscosity Phenotype Laurie.

Influenza A Subtyping The Journal of Molecular Diagnostics

The History and Impact of Molecular Coding Changes on Coverage and Reimbursement of Molecular Diagnostic Tests Susan J. Hsiao, Mahesh M. Mansukhani,

High Resolution Melting Analysis for JAK2 Exon 14 and Exon 12 Mutations Inmaculada Rapado, Silvia Grande, Enriqueta Albizua, Rosa Ayala, José-Angel Hernández,

Patrick R. Murray The Journal of Molecular Diagnostics

Detection of an Apparent Homozygous 3120G>A Cystic Fibrosis Mutation on a Routine Carrier Screen Denise LaMarche Heaney, Patrick Flume, Lauren Hamilton,

Shaoyu Zhou, Keyaunoosh Kassauei, David J. Cutler, Giulia C

Rapid Genotyping of Common MeCP2 Mutations with an Electronic DNA Microchip Using Serial Differential Hybridization William A. Thistlethwaite, Linda.

William L. Gerald, M.D., Ph.D, 1954–2008

Rapid Detection of Clonal T-Cell Receptor-β Gene Rearrangements in T-Cell Lymphomas Using the LightCycler-Polymerase Chain Reaction with DNA Melting Curve.

Quantitative Determination of JAK2 V617F by TaqMan

Brenton T. Tan, Roger A. Warnke, Daniel A. Arber

A Multicolor FISH Assay Does Not Detect DUP25 in Control Individuals or in Reported Positive Control Cells Yanina Weiland, Jürgen Kraus, Michael R. Speicher

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis Philippe Szankasi, David.

The Human Androgen Receptor X-Chromosome Inactivation Assay for Clonality Diagnostics of Natural Killer Cell Proliferations Michaël Boudewijns, Jacques.

Screening for Expanded Alleles of the FMR1 Gene in Blood Spots from Newborn Males in a Spanish Population Isabel Fernandez-Carvajal, Paulina Walichiewicz,

Olivier Gruselle, Thierry Coche, Jamila Louahed

The MECT1-MAML2 Gene Fusion and Benign Warthin's Tumor

Absence of the Caveolin-1 P132L Mutation in Cancers of the Breast and Other Organs Shinya Koike, Yasuhiro Kodera, Akimasa Nakao, Hiroji Iwata, Yasushi.

Development of a Quantitative Real-Time Polymerase Chain Reaction Assay for the Detection of the JAK2 V617F Mutation Elizabeth C. Wolstencroft, Katy.

A Practical Approach to the Detection of Prognostically Significant Genomic Aberrations in Multiple Myeloma Zhong Chen, Bonnie Issa, Shiang Huang, Emily.

The Molecular Pathology of Primary Immunodeficiencies

Comprehensive Arrayed Primer Extension Array for the Detection of 59 Sequence Variants in 15 Conditions Prevalent Among the (Ashkenazi) Jewish Population

Detection of Expanded T Cell Clones in Skin Biopsy Samples of Patients with Lichen Sclerosus et Atrophicus by T Cell Receptor-γ Polymerase Chain Reaction.

Testing for Maternal Cell Contamination in Prenatal Samples

A Rapid, Sensitive Assay to Detect EGFR Mutation in Small Biopsy Specimens from Lung Cancer Yasushi Yatabe, Toyoaki Hida, Yoshitsugu Horio, Takayuki.

A Simple Method to Confirm and Size Deletion, Duplication, and Insertion Mutations Detected by Sequence Analysis Lawrence N. Hjelm, Ephrem L.H. Chin,

Analysis of Microarray Data Using Z Score Transformation

Kathleen M. Murphy, Julie S. Cohen, Amy Goodrich, Patricia P

Design and Evaluation of a Real-Time PCR Assay for Quantification of JAK2 V617F and Wild-Type JAK2 Transcript Levels in the Clinical Laboratory Jason.

Allison M. Cushman-Vokoun, Solomon Connealy, Timothy C. Greiner

N. Scott Reading, Stephen D. Jenson, Jeffrey K. Smith, Megan S

Ye Bang-Ce, Chu Xiaohe, Fan Ye, Li Songyang, Yin Bincheng, Zuo Peng

Maria Erali, David C. Pattison, Carl T. Wittwer, Cathy A. Petti

DNA and RNA References for qRT-PCR Assays in Exfoliated Cervical Cells

Rapid and Sensitive Real-Time Polymerase Chain Reaction Method for Detection and Quantification of 3243A>G Mitochondrial Point Mutation Rinki Singh,

Multiple Sequence Variants in Hereditary Hemorrhagic Telangiectasia Cases Jamie McDonald, Friederike Gedge, Allene Burdette, James Carlisle, Changkuoth.

Amplification Refractory Mutation System, a Highly Sensitive and Simple Polymerase Chain Reaction Assay, for the Detection of JAK2 V617F Mutation in Chronic.

Katy Hanlon, Lorna W. Harries, Sian Ellard, Claudius E. Rudin

T Cell Receptor γ-Chain Gene Polymerase Chain Reaction to Diagnose Central Nervous System Involvement by Cutaneous T Cell Lymphoma Robert Taylor, Jo-Anne.

The Distribution of Gene Segments in T-Cell Receptor γ Gene Rearrangements Demonstrates the Need for Multiple Primer Sets Lyle C. Lawnicki, Ronald J.

A Real-Time PCR Assay for the Simultaneous Detection of Functional N284I and L412F Polymorphisms in the Human Toll-Like Receptor 3 Gene Robert A. Brown,

Genotyping of Human Platelet Antigens 1 to 6 and 15 by High-Resolution Amplicon Melting and Conventional Hybridization Probes Michael Liew, Lesa Nelson,

Statistical Treatment of Fluorescence in Situ Hybridization Validation Data to Generate Normal Reference Ranges Using Excel Functions Allison L. Ciolino,

Karen Snow-Bailey, Ph.D., 1961–2006

Jie Hu, Malini Sathanoori, Sally J. Kochmar, Urvashi Surti

Dominance of Nonmalignant T-Cell Clones and Distortion of the TCR Repertoire in the Peripheral Blood of Patients with Cutaneous CD30+ Lymphoproliferative.

Angiotensin I-Converting Enzyme

Monique A. Johnson, Marvin J. Yoshitomi, C. Sue Richards

Presentation transcript:

Analysis of T-Cell Receptor-γ Gene Rearrangements Using Oligonucleotide Microchip Olga A. Gra, Julia V. Sidorova, Eugene A. Nikitin, Alexander Y. Turygin, Sergey A. Surzhikov, Anait L. Melikyan, Andrey B. Sudarikov, Alexander S. Zasedatelev, Tatyana V. Nasedkina The Journal of Molecular Diagnostics Volume 9, Issue 2, Pages 249-257 (April 2007) DOI: 10.2353/jmoldx.2007.060087 Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Scheme of the microchip for detecting T-cell clonality by rearrangements of the TCR-γ locus. Gel pads with oligonucleotides complementary to sequences of the Vγ genes of family 1 (Vγ 1-8) are shown dark gray, gel pads with oligonucleotides complementary to sequences of the Vγ genes of families 2, 3, and 4 (Vγ9, Vγ10, Vγ11) are shown light gray, and gel pads with oligonucleotides complementary to sequences of the Jγ genes are shown white. Each oligonucleotide is used in duplicate on the microchip. The Journal of Molecular Diagnostics 2007 9, 249-257DOI: (10.2353/jmoldx.2007.060087) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 a: Hybridization pattern obtained on the microchip with a polyclonal sample. b: Analysis of the results. The TS is shown with a horizontal line. None of the signals exceeds the TS. The Journal of Molecular Diagnostics 2007 9, 249-257DOI: (10.2353/jmoldx.2007.060087) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 a: Hybridization pattern obtained on the microchip with a clonal sample. b: Analysis of the results suggesting a clonal rearrangement of the TCR-γ locus. The TS is shown with a horizontal line. Signals from gel pads Vγ2, Vγ10, and Jγ1/2 exceed the TS and suggest a biallelic clonal rearrangement of the TCR-γ locus with the involvement of the Vγ2, Vγ10, and Jγ1/2 genes. The Journal of Molecular Diagnostics 2007 9, 249-257DOI: (10.2353/jmoldx.2007.060087) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 a: Hybridization pattern obtained on the microchip with oligoclonal sample. b: Analysis using the threshold signal (TS) equal to <J> + 3 × σ (TS is shown with a horizontal line). c: Analysis using the TS equal to <J> + 4 × σ. The Journal of Molecular Diagnostics 2007 9, 249-257DOI: (10.2353/jmoldx.2007.060087) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 5 Sensitivity assay. Hybridization pattern (a) and analysis of the results (b) for a sample containing 10% of Jurkat cells. Signals from gel pads Vγ8, Vγ11, and Jγ1/2 exceed the TS and suggest a biallelic clonal rearrangement of the TCR-γ locus with the involvement of the Vγ8, Vγ11, and Jγ1/2 genes. Hybridization pattern (c) and analysis of the results (d) for a sample containing 5% of Jurkat cells. Only signal from gel pad Vγ11 hardly exceeds the TS; there is no sufficient data in favor of clonal rearrangement. The Journal of Molecular Diagnostics 2007 9, 249-257DOI: (10.2353/jmoldx.2007.060087) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions