Morphological alterations in pancreatic islets demonstrate a compensatory response to HFHC diet and STZ-induced β-cell loss. Morphological alterations.

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Morphological alterations in pancreatic islets demonstrate a compensatory response to HFHC diet and STZ-induced β-cell loss. Morphological alterations in pancreatic islets demonstrate a compensatory response to HFHC diet and STZ-induced β-cell loss. (A) Pancreas tissue area was quantified using morphometry software and frequency of islets expressed per centimeter squared of total pancreatic tissue (n=5 per group). HFHC-fed guinea pigs develop islet hyperplasia after 8 weeks of consuming HFHC diet, consistent with insulin resistance and a compensatory response. *P<0.05, Student's t-test. (B) Histological morphology of pancreas from an HFHC-fed guinea pig. Islets are both enlarged and more frequent compared with normal-diet controls (arrows). (C) Pancreas from a normal-diet control guinea pig demonstrates the typical frequency and size of pancreatic islets in the absence of diabetogenic treatment (arrows). Scale bar: 100 µm. (D) Representative islet morphology from a guinea pig fed HFHC diet for an extended period of 6 months. Degenerative changes are present in enlarged islets, indicated by deposition of fibrous connective tissue (arrow). Scale bar: 100 µm. (E) Islet morphology 3 weeks after STZ treatment in an HFHC/STZ guinea pig. Islets are reduced in size after treatment with STZ (arrow). Scale bar: 200 µm. (F) Immunofluorescent detection of pro-insulin (green) in an enlarged islet from an HFHC-fed guinea pig after 8 weeks of consuming the diet. Enlarged islets contain a high frequency of insulin-producing β cells. Blue is Hoechst nuclear counterstain. Scale bar: 100 µm. (G) Immunofluorescent detection of pro-insulin in an islet from an HFHC/STZ guinea pig 3 weeks after STZ treatment. There is an overall reduction in the frequency of insulin-producing β cells 3 weeks after STZ treatment. Scale bar: 100 µm. Brendan K. Podell et al. Dis. Model. Mech. 2017;10:151-162 © 2017. Published by The Company of Biologists Ltd