Time course of Fim1-GFP appearance and disappearance in actin patches.

Slides:

Advertisements

Similar presentations

The Effects of Molecular Noise and Size Control on Variability in the Budding Yeast Cell Cycle  Talia et al, Nature, 23 August 2007  William Morejón.

Advertisements

Phenotypic analysis of spo73∆ mutant.

Virulence of aggregate-forming and nonaggregate strains of Candida auris compared to C. albicans in Galleria mellonella larvae at 30°C (upper panel) and.

Colleen T. Skau, David R. Kovar Current Biology

cuf2Δ and fzr1Δ cells show a defect in meiotic termination.

Chd5 deficiency in NSCs leads to premature activation.

Janet H. Iwasa, R. Dyche Mullins Current Biology

β-CTT function requires negatively charged amino acids.

Meghal Gandhi, Vérane Achard, Laurent Blanchoin, Bruce L. Goode

The ΔrlmA mutant strain has impaired CWI pathway activation.

Volume 20, Issue 16, Pages (August 2010)

And the Dead Shall Rise: Actin and Myosin Return to the Spindle

Patient neural progenitors show a neurodevelopmental phenotype.

H-NS2 is upregulated in an hns mutant derivative of the strain E

nrde-3-dependent silencing of endogenous RNAi targets and transgenes.

Volume 25, Issue 15, Pages (August 2015)

Role of bud6p and tea1p in the interaction between actin and microtubules for the establishment of cell polarity in fission yeast Jonathan M. Glynn,

Machine-Learning-Based Analysis in Genome-Edited Cells Reveals the Efficiency of Clathrin-Mediated Endocytosis Sun Hae Hong, Christa L. Cortesio, David G.

Dibutyryl cAMP does not rescue staurosporine-induced filamentation of a cyr1Δ/cyr1Δ mutant. Dibutyryl cAMP does not rescue staurosporine-induced filamentation.

Disrupted dynamics of early stages of differentiation perturbs neural cell fate specification. Disrupted dynamics of early stages of differentiation perturbs.

PM analysis of wild-type E. coli K-12 and its rpoZ-defective mutant.

Node 42 reflected Anr activity in both existing and new experiments.

IVA can localize to and encase the forespore in the absence of VM

Hospital-level procedural sedation rates among pediatric patients in the earliest year, (2009, red circle) and the most recent year (2014, blue bar). Hospital-level.

Marko Kaksonen, Christopher P. Toret, David G. Drubin Cell

Structure and Function of an Actin-Based Filter in the Proximal Axon

Actin-Regulator Feedback Interactions during Endocytosis

KIF18A sNL variants do not accumulate on K-fibers in the center of the spindle. KIF18A sNL variants do not accumulate on K-fibers in the center of the.

Volume 21, Issue 5, Pages (March 2011)

Volume 19, Issue 23, Pages (December 2009)

Competence initiation during the progression to spore formation.

Characteristics of the Camphor-Off switch.

Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7 cells or RPE cells. Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7.

The RC4 Domain of PbrS-RNase Is Critical for Interaction with PbrActin1. The RC4 Domain of PbrS-RNase Is Critical for Interaction with PbrActin1. (A) Dissection.

Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis. Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis.

KIF18A sNL variants accumulate as well as WT on peripheral K-fibers.

TSA causes premature chromatid sister separation.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Changes throughout time in the lineage content of clones regenerated in secondary mice. Changes throughout time in the lineage content of clones regenerated.

Dictyostelium expressing N-APC lack the ability to maintain direction during migration. Dictyostelium expressing N-APC lack the ability to maintain direction.

Radial cell movement in paraxial mesoderm.

Distribution of protrusive activity.

Patterns of cell movement in paraxial mesoderm.

The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin.

No correlation between the position of the gene in the nuclear volume and the transcription levels on each of the alleles. No correlation between the position.

Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and -N media. Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and.

Fig. 4. Non-autonomous rescue of puc expression in DME cells

Mesenchymal behavior of epidermal cells in clint1 mutants.

Leukocyte infiltration of epidermis and phagocytosis of debris in clint1 mutants. Leukocyte infiltration of epidermis and phagocytosis of debris in clint1.

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

Imaging Type VI Secretion-Mediated Bacterial Killing

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

Relative growth of transcription factor knockout mutants on different sulfur sources. Relative growth of transcription factor knockout mutants on different.

Lar function is not required for Stat92E accumulation in GSCs

Precluster formation as revealed by live imaging and fixed tissue staining. Precluster formation as revealed by live imaging and fixed tissue staining.

KIF13A overexpression favors NP distribution to the cell edge and requires KIF13A binding to microtubules. KIF13A overexpressionfavorsNP distribution to.

Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons. Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Diaphanous regulates the presynaptic actin cytoskeleton.

Septin ring formation and chitin-containing septum formation are aberrant in filaments formed in response to staurosporine. Septin ring formation and chitin-containing.

TDIF-Sensitive Xylem Formation in wox4 Mutants

Volume 1, Issue 2, Pages (February 2012)

L1-expressing cells preferentially migrate into the wound edge.

Fig. 8 C9orf72 knockdown results in an increase in autophagic flux.

Hu3F8-BsAb activation of T cells.

Marko Kaksonen, Yidi Sun, David G. Drubin Cell

Response of attrappc11/rog2 Mutants to Salt Stress.

C. neoformans encodes a single family 2 DHODH required for wild-type growth. C. neoformans encodes a single family 2 DHODH required for wild-type growth.

Expression of MtrE by wild-type and mtr120 mutant strains.

Presentation transcript:

Time course of Fim1-GFP appearance and disappearance in actin patches. Time course of Fim1-GFP appearance and disappearance in actin patches. (A) Numbers of Fim1-GFP molecules over time in 95 individual actin patches in wild-type cells. (B) Mean number of Fim1-GFP molecules per actin patch over time after continuous realignment of patches in panel B. The assembly phase (blue) and disassembly phase (red) are highlighted. (C) Aligned Fim1-GFP actin patch tracks from four separate time-lapse movies of wild-type S. pombe, with assembly phases highlighted. (D) Assembly rates from the four time-lapse movies in panel D, with bar representing the mean. (E, F) Actin patch assembly rates observed in 3–4 movies of wild-type and mutant Fim1-GFP S. pombe strains. Alexander E Epstein et al. LSA 2018;1:e201800202 © 2018 Epstein et al.