Expression of bone morphogenetic proteins 4 and 7 and their receptors IA, IB, and II in human ovaries from fetuses and adults  Ronit Abir, Ph.D., Avi Ben-Haroush, M.D., M.Sc., Nir Melamed, M.D., M.Sc., Carmela Felz, Haim Krissi, M.D., Benjamin Fisch, M.D., Ph.D.  Fertility and Sterility  Volume 89, Issue 5, Pages 1430-1440 (May 2008) DOI: 10.1016/j.fertnstert.2007.04.064 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Photographs of BMP-4 expression. (A) Immunocytochemical staining of human ovary from a 27-GWs-old fetus with trisomy 21. Note the primordial follicles and the red-brown staining indicating BMP-4 expression in the oocytes (as full cytoplasmic staining without nuclear staining) and in the granuolsa and stroma cells. (B) ISH staining of human ovary from a 27-GWs-old fetus with cardiac and pulmonary abnormalities. Note the primordial follicles and the red-brown staining indicating BMP-4 expression in the oocytes (as full cytoplasmic staining without nuclear staining) and in some of the stroma cells. (C) ISH negative control of human ovary from the same fetus as in panel B. Note the primordial follicles, the overall purple-blue staining, and the lack of red-brown staining. Original magnification ×400 for all panels. Fertility and Sterility 2008 89, 1430-1440DOI: (10.1016/j.fertnstert.2007.04.064) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Photographs of BMP-7 expression. (A) Immunocytochemical staining of human ovary from a 19-year-old woman with osteosarcoma. Note the three primordial follicles with partial brown cytoplasmic and nuclear oocyte staining and expression in the stroma cells and granulosa cells indicating BMP-7 expression. (B) ISH staining of human ovary from a 22-year-old woman with leiomyosarcoma. Note the strong color in the stroma cells indicating BMP-7 expression. (C) Immunocytochemical negative control of human ovary from a 21-GWs-old fetus with hydrocephalus and limb abnormalities. Note the primordial follicles, the overall purple-blue staining, and the lack of red-brown staining. Original magnification ×400 for all panels. Fertility and Sterility 2008 89, 1430-1440DOI: (10.1016/j.fertnstert.2007.04.064) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Photographs of BMPR-IA expression. (A) Immunocytochemical staining of normal human ovary from a 27-GWs-old fetus. Note the primordial follicles and the red-brown staining indicating BMPR-IA expression in the granulosa cells and in the oocytes (as full cytoplasmic staining without nuclear staining) and in some of the stroma cells. (B) ISH staining of human ovary from a 28-GWs-old fetus with cardiac abnormalities. Note the primordial follicles and the brown staining indicating BMPR-IA expression in some of the granulosa and stroma cells and in the oocytes (as full cytoplasmic staining with nuclear staining). (C) Immunocytochemical negative control of human ovary from a 21-GWs-fetus with diaphragmatic hernia. Note the primordial follicles, the overall purple-blue staining, and the lack of brown staining. Original magnification ×400 for all panels. Fertility and Sterility 2008 89, 1430-1440DOI: (10.1016/j.fertnstert.2007.04.064) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Photographs of BMPR-IB expression. (A) Immunocytochemical staining of human ovary from a 38-year-old woman with familial breast cancer. Note the primary-secondary follicle and the brown staining indicating BMPR-IB expression in the granulosa cells, in the oocyte (as partial cytoplasmic staining without nuclear staining), and in some of the stroma cells. (B) ISH staining of human ovary from the same fetus as in Figure 3B. Note the primordial follicles and the brown staining indicating BMPR-IB expression in the granulosa cells, stroma cells, and oocytes (as full cytoplasmic staining with nuclear staining). (C) ISH negative control of human ovary from the same woman as in Figure 2A. Note the three primordial follicles, the overall purple-blue staining, and the lack of brown staining. Original magnification ×400 for all panels. Fertility and Sterility 2008 89, 1430-1440DOI: (10.1016/j.fertnstert.2007.04.064) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 5 Photographs of BMPR-II expression. (A) Immunocytochemical staining of human ovary from a 13-year-old girl with osteosarcoma. Note the primary-secondary follicle and the brown staining indicating BMPR-II expression in the granulosa cells, in the oocyte (as partial cytoplasmic staining with nuclear staining), and in some of the stroma cells. (B) ISH staining of human ovary from the same ovary as in Figure 4A. Note the atretic antral follicle and the red-brown staining indicating BMPR-II expression in the granulosa cells, stroma cells, and the follicular fluid within the antrum. (C) ISH negative control of human ovary from a 31-GWs-old fetus with brain abnormalities. Note the primordial follicles, the overall purple-blue staining, and the lack of brown staining. Original magnification ×400 for all panels. Fertility and Sterility 2008 89, 1430-1440DOI: (10.1016/j.fertnstert.2007.04.064) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions