Figure 3 Detection of JC virus (JCV) genomic DNA in mildly enlarged nuclei of oligodendroglia-like cells Detection of JC virus (JCV) genomic DNA in mildly.

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Figure 3 Detection of JC virus (JCV) genomic DNA in mildly enlarged nuclei of oligodendroglia-like cells Detection of JC virus (JCV) genomic DNA in mildly enlarged nuclei of oligodendroglia-like cells (A and B) Oligodendroglia-like cells with dot-shaped inclusions (hematoxylin-eosin). Dot-shaped inclusions were seen at the inner nuclear periphery in A (arrows), and large and small punctuated structures were present in B (arrows). (C) Immunohistochemistry against JCV capsid protein (VP2/VP3C antibody). There was only one cell suspicious for immunoreactivity against JCV capsid protein, but apparently positive cells were hardly detectable for agnoprotein. (D) In situ hybridization (ISH) for JCV genomic DNA. Sensitive ISH detected 7 oligodendroglia-like cells with JCV genomic DNA in the mildly enlarged nuclei. A–D is a serial section (scale bar: 10 μm). (E and F) PCR detection of JCV genomic DNA. DNA was extracted from either CSF specimen (E) or biopsied brain tissues (F), and the JCV-DNA copy number in each sample was determined by a quantitative PCR targeting the JCV large T gene. NC = negative control (no PCR template); PC = Positive control (approximately 100 copies/reaction). PCR were performed in duplicate (green arrows in E and red arrows in F). Shuhei Nishiyama et al. Neurol Neuroimmunol Neuroinflamm 2018;5:e415 Copyright © 2017 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.