R. Murata, M. D. , K. Nakagawa, M. D. , Ph. D. , S. Ohtori, M. D. , Ph

Slides:

Advertisements

Similar presentations

Efficacy of ultrasound therapy for the management of knee osteoarthritis: a systematic review with meta-analysis A. Loyola-Sánchez, J. Richardson, N.J.

Advertisements

The relationship between specific tissue lesions and pain severity in persons with knee osteoarthritis Ms L. Torres, B.A., Dr D.D. Dunlop, Ph.D., Dr C.

Type II collagen C2C epitope in human synovial fluid and serum after knee injury – associations with molecular and structural markers of injury N. Kumahashi,

Molecular phenotyping of HCS-2/8 cells as an in vitro model of human chondrocytes J. Saas, Ph.D., K. Lindauer, Ph.D., B. Bau, M.Sc., M. Takigawa, D.D.S.,

L. J. Sandell, Ph. D. , X. Xing, M. D. , C. Franz, M. A. , S

Mesenchymal stromal cells for cartilage repair in osteoarthritis

The epidemiology and impact of pain in osteoarthritis

Extracorporeal shock wave therapy improves motor dysfunction and pain originating from knee osteoarthritis in rats N. Ochiai, M.D., Ph.D., S. Ohtori,

Surface ultrastructure and mechanical property of human chondrocyte revealed by atomic force microscopy C.-H. Hsieh, Ph.D., Y.-H. Lin, Ph.D., S. Lin,

P.-S. Hsu, H.-H. Lin, C.-R. Li, W.-S. Chung

A. W. A. Baltzer, M. D. , C. Moser, M. D. , S. A. Jansen, M. D. , R

G.-I. Im, H.-J. Kim Osteoarthritis and Cartilage

Gene expression profiling for confirmation of effects of DNA polymeric molecules on in vitro arthritis model H.-J. Kim, A. Baek, M. Kim, S.-R. Cho Osteoarthritis.

Statistical evaluation of biomedical studies

Proteoglycan synthesis in bovine articular cartilage explants exposed to different low- frequency low-energy pulsed electromagnetic fields M. De Mattei,

Osteoarthritis year 2011 in review: biochemical markers of osteoarthritis: an overview of research and initiatives Y. Henrotin Osteoarthritis and Cartilage

K. Zou, M.D., G. Liu, M.D., T. Wu, M.D., L. Du, M.D.

Interleukin-1β and interleukin-6 disturb the antioxidant enzyme system in bovine chondrocytes: a possible explanation for oxidative stress generation

C-reactive protein, metabolic syndrome and incidence of severe hip and knee osteoarthritis. A population-based cohort study G. Engström, M.D., Ph.D.,

Characterization of mature vs aged rabbit articular cartilage: analysis of cell density, apoptosis-related gene expression and mechanisms controlling.

Glutamine protects articular chondrocytes from heat stress and NO-induced apoptosis with HSP70 expression H. Tonomura, M.D., K.A. Takahashi, M.D., Ph.D.,

Health-related quality of life and costs in patients with osteoarthritis on waiting list for total knee replacement M. Núñez, Ph.D., E. Núñez, Ph.D.,

Effect of mechanical stress on the hypertrophic differentiation related gene expression in cultured chondrocytes H. Inoue, Y. Arai, R. Terauchi, S. Nakagawa,

Efficacy and safety of intraarticular hyaluronic acid in the treatment of hip osteoarthritis: a systematic review J.C. Fernández López, M.D., Ph.D.,

Comparison of load responsiveness of cartilage T1rho and T2 in porcine knee joints: an experimental loading MRI study H. Hamada, T. Nishii, S. Tamura,

A.W. Palmer, C.G. Wilson, E.J. Baum, M.E. Levenston

Characterization of viscoelastic properties of normal and osteoarthritic chondrocytes in experimental rabbit model Q.Y. Zhang, Ph.D., X.H. Wang, M.D.,

Characterization of pro-apoptotic and matrix-degradative gene expression following induction of osteoarthritis in mature and aged rabbits Dr. C.M. Robertson,

Osteoarthritis year 2013 in review: genetics and genomics

Time to be positive about negative data?

PCB126 induces apoptosis of chondrocytes via ROS-dependent pathways

Methylation of the OP-1 promoter: potential role in the age-related decline in OP-1 expression in cartilage R.F. Loeser, M.D., H.-J. Im, Ph.D., B. Richardson,

J. Avouac, M.D., L. Gossec, M.D., M. Dougados, M.D.

Stem cell therapy for human cartilage defects: a systematic review

S. Sonne-Holm, M.D., Dr. Sci., S. Jacobsen, M.D.

Undifferentiated human mesenchymal stem cells (hMSCs) are highly sensitive to mechanical strain: transcriptionally controlled early osteo-chondrogenic.

N.D. Miljkovic, M.D., Ph.D., G.M. Cooper, Ph.D., K.G. Marra, Ph.D.

Overexpression of hsa-miR-148a promotes cartilage production and inhibits cartilage degradation by osteoarthritic chondrocytes L.A. Vonk, A.H.M. Kragten,

Osteoarthritis and Cartilage

Utilization rates of hip arthroplasty in OECD countries

Scientific production and impact of national registers: the example of orthopaedic national registers P. Boyer, I. Boutron, P. Ravaud Osteoarthritis.

Risk factors for onset of osteoarthritis of the knee in older adults: a systematic review and meta-analysis M. Blagojevic, C. Jinks, A. Jeffery, K.P.

Avocado soybean unsaponifiables (ASU) suppress TNF-α, IL-1β, COX-2, iNOS gene expression, and prostaglandin E2 and nitric oxide production in articular.

Mevastatin reduces cartilage degradation in rabbit experimental osteoarthritis through inhibition of synovial inflammation Y. Akasaki, M.D., S. Matsuda,

Who should have a joint replacement? A plea for more ‘phronesis’

Membrane type-1 matrix metalloproteinase is induced following cyclic compression of in vitro grown bovine chondrocytes J.N.A. De Croos, Ph.D., B. Jang,

Synergistic effect of chondroitin sulfate and cyclic pressure on biochemical and morphological properties of chondrocytes from articular cartilage G.

W.C. Bae, Ph.D., B.L. Schumacher, B.S., R.L. Sah, M.D., Sc.D.

N. Takahashi, Ph. D. , K. Rieneck, M. D. , P. M. van der Kraan, Ph. D

Nitric oxide decreases IGF-1 receptor function in vitro; glutathione depletion enhances this effect in vivo R.K. Studer, Ph.D. Osteoarthritis and Cartilage

D.J. Hunter Osteoarthritis and Cartilage

A single application of cyclic loading can accelerate matrix deposition and enhance the properties of tissue-engineered cartilage Dr S.D. Waldman, Ph.D.,

J. Haag, R. Voigt, S. Soeder, T. Aigner Osteoarthritis and Cartilage

Gene expression profiling of chondrocytes from a porcine impact injury model M.S. Ashwell, Ph.D., A.T. O'Nan, M.S., M.G. Gonda, Ph.D., P.L. Mente, Ph.D.

Identification and analysis of a SMAD3 cis-acting eQTL operating in primary osteoarthritis and in the aneurysms and osteoarthritis syndrome E.V.A. Raine,

S.G. Muthuri, D.F. McWilliams, M. Doherty, W. Zhang

Novel juvenile factors for cartilage regeneration

B. Zielinska, M. Killian, M. Kadmiel, M. Nelsen, T.L. Haut Donahue

R. H. J. Das, M. Sc. , H. Jahr, Ph. D. , J. A. N. Verhaar, M. D. , Ph

I. G. Otterness, Ph. D. , M. -P. H. Le Graverand, M. D. , Ph. D. , F

Dynamic compression of single cells

Osteoarthritis year 2012 in review: biology

Systematic review of the impact of osteoarthritis on health outcomes for comorbid disease in older people L. Parkinson, D.L. Waters, L. Franck Osteoarthritis.

Erratum to “Effect of hyaluronic acid (MW 500–730kDa) on proteoglycan and nitric oxide production in human osteoarthritic chondrocyte cultures exposed.

Effect of hyaluronic acid (MW 500–730kDa) on proteoglycan and nitric oxide production in human osteoarthritic chondrocyte cultures exposed to hydrostatic.

Changes in pain, stiffness and physical function in patients with osteoarthritis waiting for hip or knee joint replacement surgery H. Kapstad, R.N.,

Trapeziometacarpal subluxation predisposes to incident trapeziometacarpal osteoarthritis (OA): the Framingham study D.J. Hunter, M.B.B.S., Ph.D., Y.

M. Doherty, P. Dieppe Osteoarthritis and Cartilage

Measurement of synovial fluid volume using urea

General Information Osteoarthritis and Cartilage

Presentation transcript:

The effects of radial shock waves on gene transfer in rabbit chondrocytes in vitro R. Murata, M.D., K. Nakagawa, M.D., Ph.D., S. Ohtori, M.D., Ph.D., N. Ochiai, M.D., Ph.D., M. Arai, M.E., T. Saisu, M.D., Ph.D., T. Sasho, M.D., Ph.D., K. Takahashi, M.D., Ph.D., H. Moriya, M.D., Ph.D. Osteoarthritis and Cartilage Volume 15, Issue 11, Pages 1275-1282 (November 2007) DOI: 10.1016/j.joca.2007.04.001 Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 The relationship of pressure amplitude and relative luciferase activity (A) and cell viability (B). The cells were exposed to radial shock waves at 500 pulses and a frequency of 10Hz. Mean+SE (n=6), *P<0.05. Osteoarthritis and Cartilage 2007 15, 1275-1282DOI: (10.1016/j.joca.2007.04.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 The relationship of number of pulses and relative luciferase activity (A) and cell viability (B). The cells were exposed to radial shock waves at an amplitude of 0.3MPa and a frequency of 10Hz. Mean+SE (n=6), *P<0.05. Osteoarthritis and Cartilage 2007 15, 1275-1282DOI: (10.1016/j.joca.2007.04.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 The relationship of frequency and relative luciferase activity (A) and cell viability (B). The cells were exposed to radial shock waves at an amplitude of 0.3MPa and 500 pulses. Mean+SE (n=6), *P<0.05. Osteoarthritis and Cartilage 2007 15, 1275-1282DOI: (10.1016/j.joca.2007.04.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 The relationship of DNA concentration and relative luciferase activity (A) and cell viability (B). The cells were exposed to radial shock waves at an amplitude of 0.3MPa, 2000 pulses, and a frequency of 10Hz. Mean+SE (n=6), *P<0.05. Osteoarthritis and Cartilage 2007 15, 1275-1282DOI: (10.1016/j.joca.2007.04.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Flow cytometric analysis of GFP expression in transfected cells by radial shock waves or FuGENE6 methods. The cells treated by radial shock waves (RSW group: A) and FuGENE6 (FuGENE6 group: B) are shown. The dot plot of each group is shown as fluorescence of GFP (horizontal axis) and forward scatter (vertical axis). The cells within the sphere enclosed with a kinked line were analyzed and displayed in the histograms, respectively. In each histogram, the horizontal axis shows relative fluorescent intensity and the vertical axis shows cell numbers. Osteoarthritis and Cartilage 2007 15, 1275-1282DOI: (10.1016/j.joca.2007.04.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions