Using semen flow cytometry to evaluate association of ploidy status and chromatin condensation of spermatozoa with conventional semen parameters: Clinical.

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Using semen flow cytometry to evaluate association of ploidy status and chromatin condensation of spermatozoa with conventional semen parameters: Clinical application in intrauterine insemination Leandros Lazaros, Ph.D., Apostolos Kaponis, M.D., Georgios Vartholomatos, Ph.D., Elissavet Hatzi, Ph.D., Stefania Botsari, M.D., Nikolaos Plachouras, M.D., Georgios Makrydimas, M.D., Konstantinos Zikopoulos, M.D., Nikolaos Sofikitis, M.D., Ioannis Georgiou, Ph.D. Fertility and Sterility Volume 95, Issue 1, Pages 110-115 (January 2011) DOI: 10.1016/j.fertnstert.2010.05.012 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Association of mean ploidy rates in sperm cells with (A) sperm morphology and (B) sperm motility. Pearson correlation was used (A) between the hyperhaploidy, hypohaploidy, and diploidy rates and the percentage of normal forms of spermatozoa (P<.01) and (B) between the hyperhaploidy, hypohaploidy, haploidy, and diploidy rates and sperm motility (P<.01). Fertility and Sterility 2011 95, 110-115DOI: (10.1016/j.fertnstert.2010.05.012) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Scattergrams of acridine orange–stained spermatozoa analyzed by flow cytometry, showing (A) a semen sample containing an increased percentage of spermatozoa in red population zones and (B) a normal reference semen pool. To define the nuclear abnormalities and to estimate the percentage of mature spermatozoa in every semen sample, the data were examined using two-dimensional scattergrams plotting red versus green fluorescence. The FL1-H axis represented the green fluorescence, the FL2-H axis the red fluorescence. Fertility and Sterility 2011 95, 110-115DOI: (10.1016/j.fertnstert.2010.05.012) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Association of the mean percentage of mature spermatozoa with sperm morphology. Pearson correlation was used between the percentage of mature spermatozoa and the percentage of abnormal forms of spermatozoa (P<.01). Fertility and Sterility 2011 95, 110-115DOI: (10.1016/j.fertnstert.2010.05.012) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Association of IUI pregnancy rates with (A) the total aneuploidy rate and (B) the percentage of mature spermatozoa. Chi-square test was used to compare the positive and negative pregnancy outcomes (A) in every category of total aneuploidy rate (P<.01) and (B) in every category of percentage of mature spermatozoa (P<.01). Fertility and Sterility 2011 95, 110-115DOI: (10.1016/j.fertnstert.2010.05.012) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions