Glucosamine and chondroitin sulfate: biological response modifiers of chondrocytes under simulated conditions of joint stress L Lippiello Osteoarthritis.

Slides:

Advertisements

Similar presentations

Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen antibody-induced.

Advertisements

Variations in matrix composition and GAG fine structure among scaffolds for cartilage tissue engineering J.K. Mouw, M.S., N.D. Case, Ph.D., R.E. Guldberg,

Identification of synovial fluid microRNA signature in knee osteoarthritis: differentiating early- and late-stage knee osteoarthritis Y.-H. Li, G. Tavallaee,

Chondro-protective effects of low intensity pulsed ultrasound

J.F. Nishimuta, M.E. Levenston Osteoarthritis and Cartilage

CCN family 2/connective tissue growth factor (CCN2/CTGF) stimulates proliferation and differentiation of auricular chondrocytes T. Fujisawa, Ph.D., D.D.S.,

Moderate dynamic compression inhibits pro-catabolic response of cartilage to mechanical injury, tumor necrosis factor-α and interleukin-6, but accentuates.

IL-10 reduces apoptosis and extracellular matrix degradation after injurious compression of mature articular cartilage P. Behrendt, A. Preusse-Prange,

MAPKs are essential upstream signaling pathways in proteolytic cartilage degradation – divergence in pathways leading to aggrecanase and MMP-mediated.

Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen antibody-induced.

Depletion of gangliosides enhances cartilage degradation in mice

Infrapatellar fat pad aggravates degeneration of acute traumatized cartilage: a possible role for interleukin-6 J. He, Y. Jiang, P.G. Alexander, V. Ulici,

M. Z. Ilic, Ph. D. , B. Martinac, Ph. D. , T. Samiric, Ph. D. , C. J

Chondroitin sulphate: an effective joint lubricant?

Cannabinoid WIN-55,212-2 mesylate inhibits interleukin-1β induced matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase expression.

Nitric oxide enhances aggrecan degradation by aggrecanase in response to TNF-α but not IL-1β treatment at a post-transcriptional level in bovine cartilage.

B. Mohanraj, G.R. Meloni, R.L. Mauck, G.R. Dodge

The role of the PCM in reducing oxidative stress induced by radical initiated photoencapsulation of chondrocytes in poly(ethylene glycol) hydrogels N.

The chondroprotective effect of selective COX-2 inhibition in osteoarthritis: ex vivo evaluation of human cartilage tissue after in vivo treatment T.N.

A review of the effects of insulin-like growth factor and platelet derived growth factor on in vivo cartilage healing and repair M.B. Schmidt, Ph.D.,

Increased stromelysin-1 (MMP-3), proteoglycan degradation (3B3- and 7D4) and collagen damage in cyclically load-injured articular cartilage Peggy M.

Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell- engineered tissue constructs M. Pei, F. He, B.M. Boyce, V.L.

K.A. Payne, D.M. Didiano, C.R. Chu Osteoarthritis and Cartilage

M.A. Greene, R.F. Loeser Osteoarthritis and Cartilage

Identification of synovial fluid microRNA signature in knee osteoarthritis: differentiating early- and late-stage knee osteoarthritis Y.-H. Li, G. Tavallaee,

A.J. McGregor, B.G. Amsden, S.D. Waldman Osteoarthritis and Cartilage

L.-H. Weng, C.-J. Wang, J.-Y. Ko, Y.-C. Sun, Y.-S. Su, F.-S. Wang

S. Varghese, Ph. D. , P. Theprungsirikul, B. S. , S. Sahani, B. S. , N

Arterial heparan sulfate proteoglycans inhibit vascular smooth muscle cell proliferation and phenotype change in vitro and neointimal formation in vivo

Glucosamine promotes chondrogenic phenotype in both chondrocytes and mesenchymal stem cells and inhibits MMP-13 expression and matrix degradation A.

R. Sommaggio, M. Pérez-Cruz, J.L. Brokaw, R. Máñez, C. Costa

Interleukin-1β and interleukin-6 disturb the antioxidant enzyme system in bovine chondrocytes: a possible explanation for oxidative stress generation

Cartilage degradation independent of MMP/aggrecanases

Glucosamine reduces anabolic as well as catabolic processes in bovine chondrocytes cultured in alginate E.J. Uitterlinden, M.D., H. Jahr, Ph.D., J.L.M.

C. Candrian, S. Miot, F. Wolf, E. Bonacina, S. Dickinson, D. Wirz, M

Intra-individual comparison of human ankle and knee chondrocytes in vitro: relevance for talar cartilage repair C. Candrian, M.D., E. Bonacina, B.Sc.,

Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell- engineered tissue constructs M. Pei, F. He, B.M. Boyce, V.L.

Oxidative stress induces expression of osteoarthritis markers procollagen IIA and 3B3(−) in adult bovine articular cartilage I.M. Khan, Ph.D., S.J. Gilbert,

S.A. Nazli, R.F. Loeser, S. Chubinskaya, J.S. Willey, R.R. Yammani

Inhibition of caspase-9 reduces chondrocyte apoptosis and proteoglycan loss following mechanical trauma C.A.M. Huser, M.Sc., M. Peacock, M.E. Davies,

Osteogenic protein-1 promotes the formation of tissue-engineered cartilage using the alginate-recovered-chondrocyte method Dr. K. Masuda, M.D., B.E.

G. G. Reinholz, J. S. Fitzsimmons, M. E. Casper, T. J. Ruesink, H. W

Methylation of the OP-1 promoter: potential role in the age-related decline in OP-1 expression in cartilage R.F. Loeser, M.D., H.-J. Im, Ph.D., B. Richardson,

Resistin is elevated following traumatic joint injury and causes matrix degradation and release of inflammatory cytokines from articular cartilage in.

M. Cucchiarini, H. Madry, E.F. Terwilliger

Utility of T2 mapping and dGEMRIC for evaluation of cartilage repair after allograft chondrocyte implantation in a rabbit model J. Endo, A. Watanabe,

L. C. Davies, B. Sc. , Ph. D. , E. J. Blain, B. Sc. , Ph. D. , B

Pharmaceutical nanocarrier association with chondrocytes and cartilage explants: influence of surface modification and extracellular matrix depletion

Bisphosphonate rescues cartilage from trauma damage by promoting mechanical sensitivity and calcium signaling in chondrocytes Y. Zhou, M. Park, L. Wang,

Tissue engineering of cartilage using poly-ɛ-caprolactone nanofiber scaffolds seeded in vivo with periosteal cells M.E. Casper, J.S. Fitzsimmons, J.J.

M. Hufeland, M. Schünke, A.J. Grodzinsky, J. Imgenberg, B. Kurz

Glucosamine sulfate modulates the levels of aggrecan and matrix metalloproteinase-3 synthesized by cultured human osteoarthritis articular chondrocytes

Mevastatin reduces cartilage degradation in rabbit experimental osteoarthritis through inhibition of synovial inflammation Y. Akasaki, M.D., S. Matsuda,

Scaffold degradation elevates the collagen content and dynamic compressive modulus in engineered articular cartilage K.W. Ng, Ph.D., L.E. Kugler, B.S.,

Dynamic compression counteracts IL-1β-induced release of nitric oxide and PGE2by superficial zone chondrocytes cultured in agarose constructs T.T Chowdhury,

Spingosine-1-phosphate stimulates proliferation and counteracts interleukin-1 induced nitric oxide formation in articular chondrocytes M.H. Stradner,

Comparison between chondroprotective effects of glucosamine, curcumin, and diacerein in IL-1β-stimulated C-28/I2 chondrocytes S. Toegel, M.Pharm.S.,

Identification of molecular markers for articular cartilage

Changes in microstructure and gene expression of articular chondrocytes cultured in a tube under mechanical stress Shuitsu Maeda, M.D., Jun Nishida,

Analysis of ADAMTS4 and MT4-MMP indicates that both are involved in aggrecanolysis in interleukin-1-treated bovine cartilage P. Patwari, G. Gao, J.H.

Cartilage growth and remodeling: modulation of balance between proteoglycan and collagen network in vitro with β-aminopropionitrile A. Asanbaeva, Ph.D.,

Membrane culture and reduced oxygen tension enhances cartilage matrix formation from equine cord blood mesenchymal stromal cells in vitro C. Co, M.K.

Estrogen reduces mechanical injury-related cell death and proteoglycan degradation in mature articular cartilage independent of the presence of the superficial.

Low oxygen tension stimulates the redifferentiation of dedifferentiated adult human nasal chondrocytes1 1 Supported by IsoTis S.A. J. Malda, Ph.D., C.A.

Cartilage degeneration in different human joints

Inhibition of adenosine kinase attenuates interleukin-1- and lipopolysaccharide-induced alterations in articular cartilage metabolism Raina Petrov, B.S.,

Peroxisome proliferator activated receptor alpha activation decreases inflammatory and destructive responses in osteoarthritic cartilage S. Clockaerts,

Chondro-protective effects of low intensity pulsed ultrasound

Effects of physical stimulation with electromagnetic field and insulin growth factor-I treatment on proteoglycan synthesis of bovine articular cartilage

Effect of expansion medium on ex vivo gene transfer and chondrogenesis in type II collagen–glycosaminoglycan scaffolds in vitro R.M. Capito, Ph.D., M.

Presentation transcript:

Glucosamine and chondroitin sulfate: biological response modifiers of chondrocytes under simulated conditions of joint stress L Lippiello Osteoarthritis and Cartilage Volume 11, Issue 5, Pages 335-342 (May 2003) DOI: 10.1016/S1063-4584(03)00026-8

Fig. 1 Effect of dose and time of pronase exposure on 35-sulfate incorporation into explants of cartilage obtained from young and aged animals. (A, B, C) Explants exposed to pronase for 1, 2 and 3h, respectively. Arrows indicate those specimens, which did not differ significantly from control values. Data expressed as percent change from control values and represent mean±s.e.m. for six to eight replicates. Osteoarthritis and Cartilage 2003 11, 335-342DOI: (10.1016/S1063-4584(03)00026-8)

Fig. 2 Safranin-O-stained histologic sections of cartilage explants. (A) Control tissue; (B, C, D) explants exposed to 50U pronase for 1, 2 and 3h, respectively. Osteoarthritis and Cartilage 2003 11, 335-342DOI: (10.1016/S1063-4584(03)00026-8)

Fig. 3 Effect of CDS on incorporation of 35-sulfate by cartilage explants exposed to 50U pronase for 3h. Values representing four to six replicates are graphed as the percent change (±s.e.m.) from control explants cultured in DMEM/F-12 without enzyme treatment. All enzyme treatments significantly differed from control (no additions). Of the samples not exposed to enzyme, only 25 CDS was significantly higher (+27%) than control. Osteoarthritis and Cartilage 2003 11, 335-342DOI: (10.1016/S1063-4584(03)00026-8)

Fig. 4 Response of chondrocyte monolayers to glcN, CS and IGF-1 following heat stress. Data represent mean ±s.e.m. of six to eight replicates. All IGF-1 values and 17h at 41°C values are significantly different at P<0.001. Osteoarthritis and Cartilage 2003 11, 335-342DOI: (10.1016/S1063-4584(03)00026-8)

Fig. 5 Anticatabolic effect of CS on IL-1/plasminogen-stimulated cultures of chondrocyte monolayers. Data from eight replicates expressed as percent of total counts incorporated (±s.e.m.), which were released. Numbers reflect the total amount of radioisotope (cpm/mg dry tissue weight) in the explants at the start of the study. Arrows indicate those values, which are significantly different at P<0.05. Osteoarthritis and Cartilage 2003 11, 335-342DOI: (10.1016/S1063-4584(03)00026-8)

Fig. 6 Effect of preconditioning cartilage from young and aged bovine joints with glcN plus CS on metabolic response to static pressure. Data expressed as percent change (±s.e.m.) in specific activity of GAG (cpm/μg GAG) of 10 replicates. Young tissue inhibited by −65% with no change after CDS conditioning. Aged tissue uptake stimulated by 40% without conditioning and 1000% after conditioning. Osteoarthritis and Cartilage 2003 11, 335-342DOI: (10.1016/S1063-4584(03)00026-8)