The ER is the main source of cholesterol accumulated by STARD3 in endosomes The ER is the main source of cholesterol accumulated by STARD3 in endosomes.

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The ER is the main source of cholesterol accumulated by STARD3 in endosomes The ER is the main source of cholesterol accumulated by STARD3 in endosomes A–CHeLa/Ctrl and HeLa/STARD3 cells were incubated in normal medium (A), LPDS‐containing medium (B) or normal medium with 50 μM mevinolin and 100 μM mevalonate (C), for 48 h. Cholesterol accumulation in endosomes was detected by filipin staining (Cyan Hot) in endosomes identified by the presence of Lamp1 (red) and STARD3 (magenta). Nuclei were stained in blue. Merged image of filipin and STARD3 signals is shown in (d and h). The subpanels on the right are higher magnification (3.5×) images of the area outlined in white (a, e). The filipin and STARD3 merged image is labeled Overlay. Scale bars: 10 μm.D, ERelative fluorescence intensity of intracellular filipin in HeLa/Ctrl and HeLa/STARD3 cells incubated or not in LPDS‐containing medium (D) or treated or not with mevinolin and mevalonate (E). Mean ± SD; n = 5 (D) and n = 4 (E) independent experiments; **P < 0.01, ANOVA with Tukey's multiple comparison test. Léa P Wilhelm et al. EMBO J. 2017;36:1412-1433 © as stated in the article, figure or figure legend