Testicular and epididymal dual origin of hCAP-18/SOB3, a human sperm protein  Mireille Doussau, A.I., Andrea Lasserre, Ph.D., Sonia Hammami-Hamza, Ph.D., C. Massaad, Ph.D., Jean Marie Gasc, Ph.D., Catherine Finaz, Ph.D.  Fertility and Sterility  Volume 90, Issue 3, Pages 853-856 (September 2008) DOI: 10.1016/j.fertnstert.2007.07.1329 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Localization of hCAP-18/SOB3 mRNAs and of hCAP-18/SOB3 protein in human testis and epididymis. Hybridization with the (35S)-uridine triphosphate–labeled antisense hCAP-18/SOB3 RNA probe (A–D, G–J, M–P) and with the hCAP-18/SOB3 sense RNA probe (E, F, K, L) as a control. Bright- and dark-field images of the same sections are shown on the left and right, respectively. Arrows show early spermatogonia. Testis and epididymis sections were treated with either the anti-rec-hCAP-18/SOB3 polyclonal antibody (M, N) or with the pre-immune serum (O, P). The antibody bound to spermatocytes (sc), round spermatids (rsd), elongated spermatids (esd), and some spermatozoa (spz), whereas it did not bind to Sertoli cells (Sc). The epididymal epithelium and spermatozoa were labeled. Bars = 32 μm in A, B, E–G, H, K, and L and 8 μm in C, D, I, J, and M–P. HIS = histochemistry. Fertility and Sterility 2008 90, 853-856DOI: (10.1016/j.fertnstert.2007.07.1329) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions