2D-LC-MS/MS analysis of tryptic digest of HEK293-SUMO3 cells (2 μg inj

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2D-LC-MS/MS analysis of tryptic digest of HEK293-SUMO3 cells (2 μg inj 2D-LC-MS/MS analysis of tryptic digest of HEK293-SUMO3 cells (2 μg inj.) using DIA and targeted identification with an inclusion list. 2D-LC-MS/MS analysis of tryptic digest of HEK293-SUMO3 cells (2 μg inj.) using DIA and targeted identification with an inclusion list.A, BPC, B, XIC for m/z 243.109, C, extracted fragment ion spectrum (precursor isolation window of m/z 950 ± 50) from which the mass of the SUMO3 peptide candidate was calculated to be 2961.36 Da based on the observation of the [M+3H]3+ ion at m/z 988.1254 for the peak eluting at 34.5 min. D, MS/MS spectrum (30 V collision energy) from the targeted analysis of [M+3H]3+ at m/z 988.1234 confirming the sequence of the SUMO3 peptide as ATGDETGAK(NQTGG)VERADGYEPPVQESV corresponding to 40S ribosomal protein S3a with a modified K249 residue. Frederic Lamoliatte et al. Mol Cell Proteomics 2013;12:2536-2550 © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.