Suzanne D. Vernon, Elizabeth R. Unger, Mangalathu Rajeevan, Irina M

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Reproducibility of Alternative Probe Synthesis Approaches for Gene Expression Profiling with Arrays Suzanne D. Vernon, Elizabeth R. Unger, Mangalathu Rajeevan, Irina M. Dimulescu, Rosane Nisenbaum, Catherine E. Campbell The Journal of Molecular Diagnostics Volume 2, Issue 3, Pages 124-127 (August 2000) DOI: 10.1016/S1525-1578(10)60626-5 Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 The log base 2 values of the hybridization intensities for the triplicate experiments are plotted on a graph for each labeling method. For each scatter plot, the results of experiment 1 compared to experiment 2 are plotted in blue, and the results of experiment 1 compared to experiment 3 are plotted in red. If all results were in perfect agreement, the points would fall on the 45° identity line. The two solid parallel lines indicate the position of twofold differences in intensity. The two dashed perpendicular lines indicate the COVs. Scatter plots are shown for the three polyA probe experiments (A), the three total RNA probe experiments (B), and the three SMART probe experiments (C). The Journal of Molecular Diagnostics 2000 2, 124-127DOI: (10.1016/S1525-1578(10)60626-5) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions