Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats  Carla C. Maganhin, Ph.D., Ricardo S. Simões, Ph.D., Luiz F.P. Fuchs, Ph.D., Gisela R.S. Sasso, Ph.D., Manuel J. Simões, Ph.D., Edmund C. Baracat, Ph.D., Jose M. Soares, Ph.D.  Fertility and Sterility  Volume 102, Issue 1, Pages 291-298 (July 2014) DOI: 10.1016/j.fertnstert.2014.04.006 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Values of CYP11A1, CYP17A1, and CYP19A1 gene expression in relation to β-actin. (A) Mean and standard deviation of 2−ΔΔCt from the qRT-PCR of the CYP11A1 gene among the groups. Not a difference was detected (CYP11A1, P=.13). (B) Mean and standard deviation of 2−ΔΔCt from the qRT-PCR of the CYP17A1 gene between GI, GII, and GIII, using the same samples used in microarray experiments (CYP17A1 *P<.01 compared with GI and GIII). (C) Mean and standard deviation of 2−ΔΔCt from the qRT-PCR of the CYP19A1 gene between GI, GII, and GIII, using the same samples used in microarray experiments (CYP19A1, P<.01 compared with GI). Fertility and Sterility 2014 102, 291-298DOI: (10.1016/j.fertnstert.2014.04.006) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Photomicrographs of ovarian sections after immunostaining. Expression of Cyp11a1, Cyp17a1, and Cyp19a1. GI, control group; GII, pinealectomized; GIII, pinealectomized with melatonin replacement. Note the positive reaction of granulosa cells (*) for Cyp19a1 in the GII. Also note the intense reactivity in the theca interna (arrows) for Cyp17a1 in the GII animals. The numbers represent the respective negative controls (nonspecific goat antibodies) of immunohistochemical reactions (1) Cyp11a1; (2) Cyp17a1; (3) Cyp19a1. Bars = 20 μm; magnification ×400. Fertility and Sterility 2014 102, 291-298DOI: (10.1016/j.fertnstert.2014.04.006) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions