Estimating glucose concentration in solution

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Presentation transcript:

Estimating glucose concentration in solution Paul Beaumont (Paul.Beaumont@sserc.org.uk)

Developed by: Marjorie Smith, Dollar Funding from SAPS AstraZeneca

Aims To investigate the effect of increasing glucose concentration on the rate of reaction. To estimate the concentrations of 2 glucose solutions of unknown concentration.

∝ Glucose is a reducing sugar: MnO4- is purple, Mn2+ colourless – time taken for complete colour removal [Glu] ∝

Materials (per group) 2 small beakers White tile Stopwatch Graph paper 3 syringes (5 cm3) Indelible pen

Materials (per group) 2 small beakers White tile Stopwatch Graph paper 3 syringes (5 cm3) Indelible pen Distilled water (ca. 20 cm3) Sulfuric acid (ca. 50 cm3) Potassium permanganate (ca. 20 cm3) Glucose solutions (7 standards and 2 unknowns) 9 boiling tubes Rack

Procedure 20 cm3 of distilled water in beaker 2% glucose solution (10 cm3) Sulfuric acid (10 cm3) Potassium manganate (2 cm3)

Procedure 20 cm3 of distilled water in beaker 2% glucose solution (10 cm3) Sulfuric acid (10 cm3) Potassium manganate (2 cm3) Record the time taken for colour to completely disappear –may > min. Repeat for remaining solutions. Estimate glucose in unknowns.

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