Individual genes exhibit distinct stage-specific expression among female gametocytes. Individual genes exhibit distinct stage-specific expression among.

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Preparation of gametocytes for single-cell qPCR.

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Individual genes exhibit distinct stage-specific expression among female gametocytes. Individual genes exhibit distinct stage-specific expression among female gametocytes. Dot plots, which are ordered by PCA gene scores, show that a number of genes are more highly expressed in gametocytes collected on day 9 than in gametocytes collected on day 5. Many genes also have a bimodal distribution of gene expression on day 5. The Mann-Whitney test was used to determine the significance of gene expression differences in samples collected on day 5 versus day 9. The error bars represent the mean with the standard deviation. The mean fold change (FC) in gene expression in female gametocytes collected on day 9 and day 5 is shown for each gene. Katelyn A. Walzer et al. mSphere 2018; doi:10.1128/mSphere.00130-18