Human regulatory B cells control the TFH cell response

Slides:

Advertisements

Similar presentations

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,

Advertisements

Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,

Comparison of the allergic and nonallergic CD4+ T-cell responses to the major dog allergen Can f 1 Suvi Parviainen, MSc, Antti Taivainen, MD, PhD, Aino.

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy Yin Yao, MD, Cai-Ling.

CD39: A new surface marker of mouse regulatory γδ T cells

Sytze de Roock, PhD, Arie J

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Montelukast treatment may alter the early efficacy of immunotherapy in children with asthma Paweł Majak, MD, PhD, Błażej Rychlik, PhD, Łukasz Pułaski,

Human B cells promote T-cell plasticity to optimize antibody response by inducing coexpression of TH1/TFH signatures Jelle de Wit, PhD, Tineke Jorritsma,

Immunologic defects in 22q11.2 deletion syndrome

Regulation of TH17 cells in the mucosal surfaces

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Inhibition of differentiation, amplification, and function of human TH17 cells by intravenous immunoglobulin Mohan S. Maddur, DVM, Janakiraman Vani,

Is 9 more than 2 also in allergic airway inflammation?

Toll-like receptor 4 ligation enforces tolerogenic properties of oral mucosal Langerhans cells Jean-Pierre Allam, MD, Wen-Ming Peng, MSc, Torsten Appel,

Suzana Radulovic, MD, Mikila R. Jacobson, PhD, Stephen R

Keith J. Simons, PhD, Margherita Strolin Benedetti, PhD, F. Estelle R

Clara cell 16-kd protein downregulates TH2 differentiation of human naive neonatal T cells Sofi Johansson, MSc, Göran Wennergren, MD, PhD, Nils Åberg,

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Tacrolimus and TGF-β act synergistically on the generation of Langerhans cells Bartlomiej Kwiek, MD, Wen-Ming Peng, MSc, Jean-Pierre Allam, MD, Andrzej.

Critical role of IL-21 in modulating TH17 and regulatory T cells in Behçet disease Guillaume Geri, MSc, MD, Benjamin Terrier, MSc, MD, Michelle Rosenzwajg,

Enhanced frequency of immunoregulatory invariant natural killer T cells in the airways of children with asthma Nhan Pham-Thi, MD, Jacques de Blic, MD,

TH17 and TH22 cells: A confusion of antimicrobial response with tissue inflammation versus protection Mübeccel Akdis, MD, PhD, Oscar Palomares, PhD,

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,

Human mast cells are major IL-22 producers in patients with psoriasis and atopic dermatitis Shunya Mashiko, BPharm, Salim Bouguermouh, MD, PhD, Manuel.

Tolerogenic signaling by pulmonary CD1c+ dendritic cells induces regulatory T cells in patients with chronic obstructive pulmonary disease by IL-27/IL-10/inducible.

Benjamin T. Prince, MD, Msci, Ashley L. Devonshire, MD, MPH, Kristin A

Brent E. Palmer, PhD, Douglas G. Mack, PhD, Allison K

Time for a paradigm shift in asthma treatment: From relieving bronchospasm to controlling systemic inflammation Leif Bjermer, MD Journal of Allergy.

Role of IL-35 in sublingual allergen immunotherapy

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

Demonstration of circulating allergen-specific CD4+CD25highFoxp3+ T-regulatory cells in both nonatopic and atopic individuals Laura Maggi, BSc, Veronica.

News Beyond Our Pages Journal of Allergy and Clinical Immunology

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

What is an “eosinophilic phenotype” of asthma?

Liam O’Mahony, PhD, Mübeccel Akdis, MD, PhD, Cezmi A. Akdis, MD

H. William Kelly, PharmD Journal of Allergy and Clinical Immunology

Autophagy: Nobel Prize 2016 and allergy and asthma research

Pharmacodynamics of regulatory T cells in mice and humans treated with low-dose IL-2 Guillaume Churlaud, PharmD, PhD, Chadi Abbara, PharmD, PhD, Pierre-Axel.

News Beyond Our Pages Journal of Allergy and Clinical Immunology

IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts Séverine Létuvé, PhD, Stéphane Lajoie-Kadoch, MSc, Séverine Audusseau,

Allergen-specific CD8+ T cells in peanut-allergic individuals

Advances in the approach to the patient with food allergy

Changes in regulatory B-cell levels in bone marrow, blood, and sputum of patients with asthma following inhaled allergen challenge John-Paul Oliveria,

Mary F. Linehan, PhD, Timothy L. Frank, MD, Michelle L

Impairment of T-regulatory cells in cord blood of atopic mothers

Benjamin Chaigne, MD, Hervé Watier, MD, PhD

Epinephrine (adrenaline) absorption from new-generation, taste-masked sublingual tablets: A preclinical study Ousama Rachid, BPharm, MSc, Mutasem M.

Thymic output markers indicate immune dysfunction in DiGeorge syndrome

Wasp venom immunotherapy expands a subpopulation of CD4+CD25+ forkhead box protein 3–positive regulatory T cells expressing the T-cell receptor Vβ2 and.

Acid-suppressive drug use in pregnancy and the toddler's asthma risk: A crossover, case-control study Bianca Mulder, BSc, Catharina Carolina Maria Schuiling-Veninga,

Human basophils may not undergo modulation by DC-SIGN and mannose receptor– targeting immunotherapies due to absence of receptors Mrinmoy Das, MSc, Caroline.

Ovalbumin-specific IgE modulates ovalbumin-specific T-cell response after repetitive oral antigen administration Nemuko Omata, MD, Yusei Ohshima, MD,

Macrolide antibiotics and asthma treatment

Asthma: The past, future, environment, and costs

Julie Negri, BA, S. Brandon Early, BA, John W

Janet Chou, MD, Maxim Lutskiy, MD, PhD, Erdyni Tsitsikov, PhD, Luigi D

IgG4 production is confined to human IL-10–producing regulatory B cells that suppress antigen-specific immune responses Willem van de Veen, MSc, Barbara.

Environmental factors and eosinophilic esophagitis

The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.

News Beyond Our Pages Journal of Allergy and Clinical Immunology

Advances and highlights in mechanisms of allergic disease in 2015

Natural history of cow’s milk allergy

Alexander S. Kim, MD, Taylor A. Doherty, MD, Maya R

Primary prevention of asthma and allergy

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Comparison of the allergic and nonallergic CD4+ T-cell responses to the major dog allergen Can f 1 Suvi Parviainen, MSc, Antti Taivainen, MD, PhD, Aino.

Der p 1–induced CD4+FOXP3+GATA3+ T cells have suppressive properties and contribute to the polarization of the TH2-associated response Lieke Reubsaet,

Presentation transcript:

Human regulatory B cells control the TFH cell response Achouak Achour, PhD, Quentin Simon, PhD, Audrey Mohr, MSc, Jean-François Séité, PhD, Pierre Youinou, MD, PhD, Boutahar Bendaoud, PharmD, Ibtissem Ghedira, PharmD, PhD, Jacques-Olivier Pers, DDS, PhD, Christophe Jamin, PhD Journal of Allergy and Clinical Immunology Volume 140, Issue 1, Pages 215-222 (July 2017) DOI: 10.1016/j.jaci.2016.09.042 Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Journal of Allergy and Clinical Immunology 2017 140, 215-222DOI: (10 Journal of Allergy and Clinical Immunology 2017 140, 215-222DOI: (10.1016/j.jaci.2016.09.042) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Breg cells inhibit TFH cell development but not differentiated T cells. A and B, Expression of TFH cell markers on CD4+ stimulated T cells cocultured with Breg cells (Fig 1, A) or nonstimulated B cells (Fig 1, B). C, Percentage of cells from purified CD4+ T cells. D, Expression of all markers on already differentiated TFH cells cocultured with Breg cells. *P < .05 and **P < .01 (Wilcoxon test). Results are means ± SEMs of 6 experiments. Journal of Allergy and Clinical Immunology 2017 140, 215-222DOI: (10.1016/j.jaci.2016.09.042) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Characterization of Breg cell–suppressive activity on TFH cell development. A, Expression and percentages at day 5 of TFH cell markers on stimulated T cells cocultured in a Transwell (TW) system. B and C, Time course (Fig 2, B) and percentages (Fig 2, C) with Breg cells or their supernatant (spt). D and E, Percentages after coculture with blocking antibodies. *P < .05, **P < .01, and ***P < .001 (Wilcoxon test). Results are means ± SEMs of 6 experiments. Journal of Allergy and Clinical Immunology 2017 140, 215-222DOI: (10.1016/j.jaci.2016.09.042) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Breg cells suppress TFH cell function. A, Frequencies of CD138+CD19+ plasma cells and CD19+IgD−CD27+ memory B cells during cocultures of B cells and TFH cells with Breg cells. B and C, Concentration of immunoglobulins in supernatants (Fig 3, B) and after cocultures with TFH cells from purified naive and memory T cells (Fig 3, C). TW, Transwell system. *P < .05, **P < .01, ***P < .001, and ****P < .0001 (Wilcoxon test). Results are means ± SEMs of 6 experiments. Journal of Allergy and Clinical Immunology 2017 140, 215-222DOI: (10.1016/j.jaci.2016.09.042) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Characterization of Breg cell–suppressive effects on TFH cell function. A, IL-21R expression on T and B cells. B, Percentages of Foxp3+ T cells. C, Secretion of TGF-β and IL-10. D, Percentages of Foxp3+ T cells among CXCR5+PD-1+ T cells after coculture with blocking antibodies or in Transwell (TW) system. *P < .05, **P < .01, ***P < .001, and ****P < .0001 (Wilcoxon test). Results are means ± SEMs of 6 experiments. Journal of Allergy and Clinical Immunology 2017 140, 215-222DOI: (10.1016/j.jaci.2016.09.042) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Breg cells suppress the function of in vivo–differentiated T cells. A, Concentration of immunoglobulins after cocultures of B cells with in vivo–differentiated CXCR5highPD-1high TFH cells or CXCR5−PD-1− T and Breg cells. B and C, Expression of TFH cell markers on sorted T cells after coculture. D, Percentages of Foxp3+ T cells among the sorted T cells after cocultures. *P < .05 and **P < .01 (Wilcoxon and Mann-Whitney tests). Results are means ± SEMs of 6 experiments. Journal of Allergy and Clinical Immunology 2017 140, 215-222DOI: (10.1016/j.jaci.2016.09.042) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions