Volume 63, Issue 6, Pages 2162-2170 (June 2003) Hypertension superimposed on type II diabetes in Goto Kakizaki rats induces progressive nephropathy Ulf Janssen, Stephen G. Riley, Athina Vassiliadou, Jürgen Floege, Aled O. Phillips Kidney International Volume 63, Issue 6, Pages 2162-2170 (June 2003) DOI: 10.1046/j.1523-1755.2003.00007.x Copyright © 2003 International Society of Nephrology Terms and Conditions
Figure 1 Systolic blood pressure in Goto Kakizaki (GK) (A) and Wistar rats (B) 1 week before and every 2 weeks after start of treatment with deoxycorticosterone acetate (DOCA) salt (■), vehicle salt (▴), or DOCA tap water (▾).*P < 0.05 vs. control. Kidney International 2003 63, 2162-2170DOI: (10.1046/j.1523-1755.2003.00007.x) Copyright © 2003 International Society of Nephrology Terms and Conditions
Figure 2 Proteinuria in Goto Kakizaki (GK) (A) and Wistar rats (B) 1 week before and every 4 weeks after start of treatment with deoxycorticosterone acetate (DOCA) salt (■), vehicle salt (▴), or DOCA tap water (▾).*P < 0.05 vs. controls; **P < 0.05 vs. DOCA salt–treated Wistar rats and controls. Kidney International 2003 63, 2162-2170DOI: (10.1046/j.1523-1755.2003.00007.x) Copyright © 2003 International Society of Nephrology Terms and Conditions
Figure 3 Glomerular histologic findings in Goto Kakizaki (GK) and Wistar rats 15 and 24 weeks after start of treatment with DOCA salt (■), vehicle-salt (), or DOCA-tap water (□). Quantitative assessment of glomerulosclerosis (%) (A). Immunostaining scores for type IV collagen (B), α-smooth muscle actin (α-SMA) (C), and desmin (D). Monocyte/macrophage influx: counts of ED1-positive monocytes/macrophages (E). Cell proliferation: counts of BrdU-positive cells (F). ND = not determined. Kidney International 2003 63, 2162-2170DOI: (10.1046/j.1523-1755.2003.00007.x) Copyright © 2003 International Society of Nephrology Terms and Conditions
Figure 4 Renal desmin immunostaining. (A) A nonhypertensive Goto Kakizaki (GK) control rat 24 weeks after start of treatment with vehicle salt. Desmin expression is confined to the mesangium. (B) A hypertensive Wistar rat 24 weeks after start of treatment with deoxycorticosterone acetate (DOCA) salt. Desmin expression mostly exhibits a normal pattern in that it is confined to the mesangium. However, focal up-regulation is present in two podocytes at the edge of the glomerular tuft (arrows). (C) A hypertensive GK rat 24 weeks after start of treatment with DOCA salt. Glomerular desmin expression is markedly up-regulated predominantely at the glomerular edge. Kidney International 2003 63, 2162-2170DOI: (10.1046/j.1523-1755.2003.00007.x) Copyright © 2003 International Society of Nephrology Terms and Conditions
Figure 5 Electron microscopy findings. (A) Hypertensive Goto Kakizaki (GK) rat 24 weeks after start of treatment with deoxycorticosterone acetate (DOCA) salt. This shows podocytes with foot process effacement (arrowheads) as well as dense bodies (arrows). (B) Hypertensive GK rat 24 weeks after start of treatment with DOCA salt. Podocytes with microvilli formation (arrowheads) and lamella bodies (arrows). Kidney International 2003 63, 2162-2170DOI: (10.1046/j.1523-1755.2003.00007.x) Copyright © 2003 International Society of Nephrology Terms and Conditions
Figure 6 Tubulointerstitial histologic findings in Goto Kakizaki (GK) and Wistar rats 15 and 24 weeks after start of treatment with deoxycorticosterone (DOCA) salt (■), vehicle salt (), or DOCA tap water (□). Quantitative assessment of tubulointerstitial damage (A). Immunostaining scores for type IV collagen (B) and α-smooth muscle actin (α-SMA) (C). Monocyte/macrophage influx: counts of ED1-positive monocytes/macrophages (D). Cell proliferation: counts of BrdU-positive cells (E). Immunostaining score for monocyte chemoattractant protein-1 (MCP-1) (F). ND = not determined. Kidney International 2003 63, 2162-2170DOI: (10.1046/j.1523-1755.2003.00007.x) Copyright © 2003 International Society of Nephrology Terms and Conditions