Increases in phosphorylation of SAPK/JNK and p38MAPK correlate negatively with mouse embryo development after culture in different media Yingchun Wang,

Slides:

Advertisements

Similar presentations

Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice Zaraq Khan, M.B.B.S., Heather S.

Advertisements

Expression of the novel gene embryo implantation factor 2 (EMO2) in the mouse uterus at the implantation sites Zhaogui Sun, Ph.D., Renwei Su, Ph.D.,

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Lynn B. Davis, M. D. , M. S. , Sara J. Champion, M. S. , Steve O

Multiple gestation associated with infertility therapy: an American Society for Reproductive Medicine Practice Committee opinion Fertility and Sterility

Krinos M. Trokoudes, M. D. , Constantinos Pavlides, M. Sc

Demián Glujovsky, M.D., M.Sc., Cynthia Farquhar, M.D., M.P.H.

A giant oocyte in a cohort of retrieved oocytes: does it have any effect on the in vitro fertilization cycle outcome? Ronit Machtinger, M.D., Joseph.

Lynn B. Davis, M. D. , M. S. , Sara J. Champion, M. S. , Steve O

Gene expression analysis of a new source of human oocytes and embryos for research and human embryonic stem cell derivation Sharon F. Sneddon, Ph.D.,

Iron overload–modulated nuclear factor kappa-B activation in human endometrial stromal cells as a mechanism postulated in endometriosis pathogenesis

Local injury to the endometrium in controlled ovarian hyperstimulation cycles improves implantation rates Liang Zhou, M.D., Rong Li, M.D., Ph.D., Rong.

Daily physical activity and hot flashes in the Study of Women's Health Across the Nation (SWAN) Flashes Study Carolyn Gibson, M.P.H., M.S., Karen Matthews,

Karl R. Hansen, M. D. , Ph. D. , George M. Hodnett, M. D

Patient and cycle characteristics predicting high pregnancy rates with single-embryo transfer: an analysis of the Society for Assisted Reproductive Technology.

Fernando Zegers-Hochschild, M.D. Fertility and Sterility

Michael M. Alper, M.D. Fertility and Sterility

Composition of single-step media used for human embryo culture

Yu Wang, M. D. , Ph. D. , Yang Lv, Ph. D. , Liyan Wang, M. D

Adherence compounds in embryo transfer media for assisted reproductive technologies: summary of a Cochrane review Stephan Bontekoe, M.D., Neil Johnson,

Contrasting patterns in in vitro fertilization pregnancy rates among fresh autologous, fresh oocyte donor, and cryopreserved cycles with the use of day.

Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study Nicole Hope, M.B., B.S., Hon., Luk.

A prospective randomized controlled trial of preimplantation genetic screening in the “good prognosis” patient Liza R. Meyer, M.S., Sigal Klipstein,

Long-term liquid nitrogen vapor storage of mouse embryos cryopreserved using vitrification or slow cooling Jin Hee Eum, M.Sc., Jae Kyun Park, M.Sc.,

The relative contribution of assisted reproductive technologies and ovulation induction to multiple births in the United States 5 years after the Society.

Live birth of twins derived from zona-free oocytes

Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice Zaraq Khan, M.B.B.S., Heather.

Iris G. Insogna, M. D. , Malinda S. Lee, M. D. , M. B. A. , Rebecca M

Predictive value of embryo grading for embryos with known outcomes

Proteomic analysis of individual human embryos to identify novel biomarkers of development and viability Mandy G. Katz-Jaffe, Ph.D., David K. Gardner,

Martin D. Keltz, M. D. , Josh C. Skorupski, M. D. , Katrina Bradley, M

Oocyte cryopreservation

The effect of blastomere biopsy on preimplantation mouse embryo development and global gene expression Francesca E. Duncan, Ph.D., Paula Stein, Ph.D.,

Expression of the novel gene embryo implantation factor 2 (EMO2) in the mouse uterus at the implantation sites Zhaogui Sun, Ph.D., Renwei Su, Ph.D.,

Reply of the Authors Fertility and Sterility

Estrogen receptor β agonist diarylpropionitrile inhibits lipopolysaccharide-induced regulated on activation normal T cell expressed and secreted (RANTES)

Composition of commercial media used for human embryo culture

Influence of acupuncture stimulation on pregnancy rates for women undergoing embryo transfer Caroline Smith, Ph.D., Meaghan Coyle, B.Hlth.Sc. (Acup.),

Activating transcription factor 3 gene expression suggests that tissue stress plays a role in leiomyoma development Mark Payson, M.D., Minnie Malik,

Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis Li-Ying Yan, Ph.D., Jie Qiao, M.D., Yuan.

Tomer Singer, M. D. , David H. Barad, M. D. , M. S

Management of tubal ectopic pregnancy: methotrexate and salpingostomy are preferred to preserve fertility Stephanie Beall, M.D., Ph.D., Alan H. DeCherney,

Clomiphene citrate at 50: the dawning of assisted reproduction

Normal birth after single-embryo transfer in a patient with excessive polypronuclear zygote formation following in vitro fertilization and intracytoplasmic.

Anil Dubey, Ph. D. , Molina B. Dayal, M. D. , M. P. H

Differential pH in embryo culture

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Fei Wang, M. D. , Ph. D. , Zeqing Wen, M. D. , Hui Li, Ph. D

Nitric oxide metabolite production in the human preimplantation embryo and successful blastocyst formation Christopher W. Lipari, M.D., Jairo E. Garcia,

Matthew A. Will, M. D. , Murugesan Palaniappan, Ph. D

“Information-rich” reproductive outcomes in carriers of a structural chromosome rearrangement ascertained on the basis of recurrent pregnancy loss Michelle.

Human embryo twinning with applications in reproductive medicine

Immunofluorescence Localization Of Epithelial Cadherin, Cross Reactive With An Antibody Against Human Placental Protein, In Human Preimplantation Embryos

Selective insulin-like growth factor-I antagonist inhibits mouse embryo development in a dose-dependent manner Jose Inzunza, Ph.D., Olle Danielsson,

Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D

Pregnancy outcome in infertile patients with polycystic ovary syndrome who were treated with metformin Samuel S. Thatcher, M.D., Ph.D., Elizabeth M.

L. Scott, J. Bernsten, K. DeLegge, J. Hill, N. Ramsing

Fertility and Sterility: an evaluation

Insulin growth factors may be implicated in human sperm capacitation

Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells Tadahiro Yasuo, M.D., Kotaro Kitaya, M.D. Fertility.

Yoko Kumasako, B. E. , Eiko Otsu, M. En. , Takafumi Utsunomiya, M. D

Current evaluation of amenorrhea

Genetic evaluation procedures at sperm banks in the United States

Susanna McReynolds, Ph. D. , Monika Dzieciatkowska, Ph. D

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

In vitro sildenafil citrate use as a sperm motility stimulant

Krinos M. Trokoudes, M. D. , Constantinos Pavlides, M. Sc

Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization.

P-24 Fertility and Sterility Volume 85, Pages S19-S20 (April 2006)

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Presentation transcript:

Increases in phosphorylation of SAPK/JNK and p38MAPK correlate negatively with mouse embryo development after culture in different media Yingchun Wang, M.D., Ph.D., Elizabeth E. Puscheck, M.D., M.S., Jennifer J. Lewis, M.S., Anna B. Trostinskaia, M.D., Ph.D., Fangfei Wang, M.D., Daniel A. Rappolee, Ph.D. Fertility and Sterility Volume 83, Issue 4, Pages 1144-1154 (April 2005) DOI: 10.1016/j.fertnstert.2004.08.038 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 p38MAPK phosphorylation is detected more intensely in preimplantation embryos (E3.5) cultured for 24 hours in Ham's F10+BSA (A) than in QCM (B). In B, the bar indicates 100 μM. Fertility and Sterility 2005 83, 1144-1154DOI: (10.1016/j.fertnstert.2004.08.038) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Stress-activated protein kinase/Jun kinase (SAPK/JNK) phosphorylation is detected more intensely in preimplantation embryos (E3.5) cultured for 24 hours in M-16 (A) than in QCM (B). In B, the bar indicates 100 μM. Fertility and Sterility 2005 83, 1144-1154DOI: (10.1016/j.fertnstert.2004.08.038) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 Correlation of E1.5 embryo preimplantation development during 72-hour culture in different media with the SAPK/JNK phosphorylation fluorescent intensity level. A, 4-cell/compaction rate (Pearson correlation, r = −0.791, P<.05); B, morula formation rate (r = −0.855, P<.05); C, blastocyst formation rate (r = −0.899, P<.01); D, hatching rate (r = −0.808, P<.05). Fertility and Sterility 2005 83, 1144-1154DOI: (10.1016/j.fertnstert.2004.08.038) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 4 Western blot analysis of E3.5 embryo ex vivo, cultured for 24 hours in Ham's F10+BSA and KSOM+AA. Mouse embryo isolated on E3.5 tested for p38MAPK all forms and phosphorylation, SAPK/JNK all forms, and phosphorylation expression by Western blot analysis (described in the Materials and Methods section) showed correct sized bands at 43 kDa for p38MAPK and 46 and 54 kDa for SAPK/JNK. Both SAPK/JNK and p38MAPK phosphorylation was greatly increased when embryos were cultured for 24 hours in Ham's F10, compared with KSOM+AA. Ham's F10 vs. KSOM+AA: SAPK/JNK phospho/SAPK/JNK all forms = 1.45, 0.78; p38MAPK phospho/p38MAPK all forms = 1.15, 0.62. Note that 80–110 embryos were used in all groups, but some loss of embryo lysate may occur (for example, Ham's F10+BSA group for p38MAPK). Histograms represent SAPK phosphorylation or p38MAPK phosphorylation normalized to all forms. Fertility and Sterility 2005 83, 1144-1154DOI: (10.1016/j.fertnstert.2004.08.038) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions