The New Aryl Hydrocarbon Receptor Antagonist E/Z-2-Benzylindene-5,6-Dimethoxy- 3,3-Dimethylindan-1-One Protects against UVB-Induced Signal Transduction

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Presentation transcript:

The New Aryl Hydrocarbon Receptor Antagonist E/Z-2-Benzylindene-5,6-Dimethoxy- 3,3-Dimethylindan-1-One Protects against UVB-Induced Signal Transduction Julia Tigges, Thomas Haarmann-Stemmann, Christoph F.A. Vogel, Annemarie Grindel, Ulrike Hübenthal, Heidi Brenden, Susanne Grether-Beck, Gabriele Vielhaber, William Johncock, Jean Krutmann, Ellen Fritsche Journal of Investigative Dermatology Volume 134, Issue 2, Pages 556-559 (February 2014) DOI: 10.1038/jid.2013.362 Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Characterization of antagonistic capacities of E/Z-2-benzylidene-5,6-dimethoxy-3,3-dimethylindan-1-one (BDDI) in normal human epidermal keratinocytes (NHEKs). (a) Chemical structure of BDDI. (b) Effect of BDDI on cell viability (CellTiter Blue; CTB) and cytotoxicity (lactate dehydrogenase; LDH). The black line marks the respective control level. (c) Effect of BDDI on B(a)P (250 nM for 4 hours)-, 6-formylindolo[3,2-b]carbazole (FICZ; 100 nM for 4 hours)-, and UVB (100 J m−2 for 8 hours)-induced CYP1A1 mRNA expression (quantitative reverse transcription-PCR data). (d) Effect of BDDI on 7-O-ethoxyresorufin-deethylase (EROD) activity induced by 24 hours B(a)P shown in activity as pmol min−1−mg−1. (e) Transient effect of BDDI pretreatment (1 and 24 hours) on CYP1A1 mRNA expression induced by 100 J m−2 UVB. (f) NHEKs were irradiated with 100 J m−2 UVB and directly after irradiation treated with 3.3 and 33 μM BDDI. After 8 hours, RNA was isolated, and CYP1A1 transcription was analyzed. Each graph represents mean±SEM of three independent experiments; *P<0.05 versus medium/DMSO control; #P<0.05 versus treatment (B(a)P, FICZ, or UVB, respectively). Journal of Investigative Dermatology 2014 134, 556-559DOI: (10.1038/jid.2013.362) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 BDDI disturbs XRE binding of aryl hydrocarbon receptor (AhR)/ARNT and represses UVB-induced gene expression in a human in vivo study. (a) HaCaT keratinocytes were treated with 10 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 100 nM 6-formylindolo[3,2-b]carbazole (FICZ), 10 μM 3′-methoxy-4′-nitroflavone (MNF), and/or 3.3 μM BDDI for 90 minutes before isolation of nuclear extracts and were hybridized with a xenobiotic-responsive element (XRE) consensus oligonucleotide. A representative electrophoretic mobility shift assay of three independent experiments is shown. (b) Volunteers were pretreated with 0.5% BDDI or placebo for 4 days every day followed by UVB irradiation (1.5 minimal erythema dose, MED) on day 4. Twenty-four hours after irradiation, 4-mm skin biopsies were taken, RNA was isolated and reverse transcribed, and mRNA expression of CYP1A1, cyclooxygenase-2, and matrix metalloproteinase-1 was measured. Gene expression in sham-irradiated, untreated control skin were arbitrarily set as 1; n=10, mean±SEM, *P<0.001 versus unirradiated control, #P<0.001 versus UVB (1.5 MED). Journal of Investigative Dermatology 2014 134, 556-559DOI: (10.1038/jid.2013.362) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions