Quantitative Amplification of Genomic DNA from Histological Tissue Sections after Staining with Nuclear Dyes and Laser Capture Microdissection Torsten.

Slides:

Advertisements

Similar presentations

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Advertisements

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

High-Quality Genotyping Data from Formalin-Fixed, Paraffin-Embedded Tissue on the Drug Metabolizing Enzymes and Transporters Plus Array Hanneke I. Vos,

Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues Martina Doleshal,

Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers Rebecca A. Betensky, Catherine.

Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues Martina Doleshal,

Genotyping of DNA Samples Isolated from Formalin-Fixed Paraffin-Embedded Tissues Using Preamplification Renee Baak-Pablo, Vincent Dezentje, Henk-Jan.

Sensitive Detection of KRAS Mutations in Archived Formalin-Fixed Paraffin-Embedded Tissue Using Mutant-Enriched PCR and Reverse-Hybridization Christoph.

Chromogenic In Situ Hybridization for α6β4 Integrin in Breast Cancer

RT-PCR Analysis of RNA Extracted from Bouin-Fixed and Paraffin-Embedded Lymphoid Tissues Annunziata Gloghini, Barbara Canal, Ulf Klein, Luigino Dal Maso,

Volume 61, Issue 2, Pages (February 2002)

Detection of Low-Level KRAS Mutations Using PNA-Mediated Asymmetric PCR Clamping and Melting Curve Analysis with Unlabeled Probes Ji Eun Oh, Hee Sun.

Application of Selected Reaction Monitoring for Multiplex Quantification of Clinically Validated Biomarkers in Formalin-Fixed, Paraffin-Embedded Tumor.

A Quantitative Reverse Transcriptase-Polymerase Chain Reaction Assay to Identify Metastatic Carcinoma Tissue of Origin Dimitri Talantov, Jonathan Baden,

Whole Genome Amplification for Array Comparative Genomic Hybridization Using DNA Extracted from Formalin-Fixed, Paraffin-Embedded Histological Sections

Hendrikus J. Dubbink, Peggy N. Atmodimedjo, Ronald van Marion, Niels M

Mark G. Erlander, Xiao-Jun Ma, Nicole C

Separate Quality-Control Measures Are Necessary for Estimation of RNA and Methylated DNA from Formalin-Fixed, Paraffin-Embedded Specimens by Quantitative.

Comparison of Snap Freezing versus Ethanol Fixation for Gene Expression Profiling of Tissue Specimens Mark A. Perlmutter, Carolyn J.M. Best, John W.

Cold-Temperature Plastic Resin Embedding of Liver for DNA- and RNA-Based Genotyping Sydney D. Finkelstein, Rajiv Dhir, Mordechai Rabinovitz, Michelle.

Successful Application of Microarray Technology to Microdissected Formalin-Fixed, Paraffin-Embedded Tissue Renata A. Coudry, Sibele I. Meireles, Radka.

Analysis of T-Cell Clonality Using Laser Capture Microdissection and High-Resolution Microcapillary Electrophoresis Evgeny Yakirevich, Cynthia L. Jackson,

Immunoguided Laser Assisted Microdissection Techniques for DNA Methylation Analysis of Archival Tissue Specimens Franziska C. Eberle, Jeffrey C. Hanson,

Development and Validation of a Preanalytic Procedure for Performing the cobas HPV Test in SurePath Preservative Fluid Mark D. Krevolin, David Hardy,

Maria J. Worsham, Sandra R. Wolman, Richard J. Zarbo

IgH PCR of Zinc Formalin-Fixed, Paraffin-Embedded Non-Lymphomatous Gastric Samples Produces Artifactual “Clonal” Bands Not Observed in Paired Tissues.

Simultaneous Isolation of DNA and RNA from the Same Cell Population Obtained by Laser Capture Microdissection for Genome and Transcriptome Profiling

Quantitative Expression Profiling in Formalin-Fixed Paraffin-Embedded Samples by Affymetrix Microarrays Diana Abdueva, Michele Wing, Betty Schaub, Timothy.

Maxim B. Freidin, Neesa Bhudia, Eric Lim, Andrew G

The Effect of Primer-Template Mismatches on the Detection and Quantification of Nucleic Acids Using the 5′ Nuclease Assay Ralph Stadhouders, Suzan D.

RCL2, a New Fixative, Preserves Morphology and Nucleic Acid Integrity in Paraffin- Embedded Breast Carcinoma and Microdissected Breast Tumor Cells Christophe.

Quantitative mRNA Expression Analysis from Formalin-Fixed, Paraffin-Embedded Tissues Using 5′ Nuclease Quantitative Reverse Transcription-Polymerase Chain.

The somatic GNAQ mutation (R183Q) is primarily located within the blood vessels of port wine stains Wenbin Tan, PhD, Dawnica Mercado Nadora, BS, Lin.

Sensitive Detection of KRAS Mutations in Archived Formalin-Fixed Paraffin-Embedded Tissue Using Mutant-Enriched PCR and Reverse-Hybridization Christoph.

Enhancing techniques to evaluate tumor margins

Getting Things Backwards to Prevent Primer Dimers

Combined Use of PCR-Based TCRG and TCRB Clonality Tests on Paraffin-Embedded Skin Tissue in the Differential Diagnosis of Mycosis Fungoides and Inflammatory.

Low Copy Number DNA Template Can Render Polymerase Chain Reaction Error Prone in a Sequence-Dependent Manner Mansour Akbari, Marianne Doré Hansen, Jostein.

High-Quality Genotyping Data from Formalin-Fixed, Paraffin-Embedded Tissue on the Drug Metabolizing Enzymes and Transporters Plus Array Hanneke I. Vos,

Evaluation of BRAF Mutation Testing Methodologies in Formalin-Fixed, Paraffin- Embedded Cutaneous Melanomas Johanne Lade-Keller, Kirsten M. Rømer, Per.

Gene Expression Screening of Salivary Gland Neoplasms

Capillary Electrophoresis Artifact Due to Eosin

Diagnostic Utility of Molecular Investigation in Extraskeletal Myxoid Chondrosarcoma Stefania Benini, Stefania Cocchi, Gabriella Gamberi, Giovanna Magagnoli,

The MECT1-MAML2 Gene Fusion and Benign Warthin's Tumor

The Molecular Pathology of Primary Immunodeficiencies

Accurate and Reproducible Gene Expression Profiles from Laser Capture Microdissection, Transcript Amplification, and High Density Oligonucleotide Microarray.

An Array-Based Analysis of MicroRNA Expression Comparing Matched Frozen and Formalin-Fixed Paraffin-Embedded Human Tissue Samples Xiao Zhang, Jiamin.

Amplification Patterns of Three Genomic Regions Predict Distant Recurrence in Breast Carcinoma Lisa M. Davis, Cole Harris, Lei Tang, Patti Doherty, Peter.

Validation of a Gene Expression Test for Mesothelioma Prognosis in Formalin-Fixed Paraffin-Embedded Tissues Assunta De Rienzo, Robert W. Cook, Jeff Wilkinson,

A Novel Approach to Detect Programed Death Ligand 1 (PD-L1) Status and Multiple Tumor Mutations Using a Single Non–Small-Cell Lung Cancer (NSCLC) Bronchoscopy.

The Pitfalls of Companion Diagnostics

Successful Application of Hyperbranched Multidisplacement Genomic Amplification to Detect HIV-1 Sequences in Single Neurons Removed from Autopsy Brain.

David L. Evers, Junkun He, Yeon Ho Kim, Jeffrey T. Mason, Timothy J

Comprehensive Genomic Profiling of Malignant Effusions in Patients with Metastatic Lung Adenocarcinoma Soo-Ryum Yang, Chieh-Yu Lin, Henning Stehr, Steven.

Low Incidence of Minor BRAF V600 Mutation-Positive Subclones in Primary and Metastatic Melanoma Determined by Sensitive and Quantitative Real-Time PCR

Gayatry Mohapatra, Rebecca A. Betensky, Ezra R

Beatrice S. Knudsen, April N. Allen, Dale F. McLerran, Robert L

Cecily P. Vaughn, Elaine Lyon, Wade S. Samowitz

JAZF1/JJAZ1 Gene Fusion in Endometrial Stromal Sarcomas

Improved Detection of KIT Exon 11 Duplications in Formalin-Fixed, Paraffin-Embedded Gastrointestinal Stromal Tumors Jerzy Lasota, Bartosz Wasag, Sonja.

A Novel Method to Compensate for Different Amplification Efficiencies between Patient DNA Samples in Quantitative Real-Time PCR Jules Meijerink, Caroline.

Custom Design of a GeXP Multiplexed Assay Used to Assess Expression Profiles of Inflammatory Gene Targets in Normal Colon, Polyp, and Tumor Tissue Janice.

Validation and Reproducibility of a Microarray-Based Gene Expression Test for Tumor Identification in Formalin-Fixed, Paraffin-Embedded Specimens Raji.

Improved Detection of the KIT D816V Mutation in Patients with Systemic Mastocytosis Using a Quantitative and Highly Sensitive Real-Time qPCR Assay Thomas.

Monique A. Johnson, Marvin J. Yoshitomi, C. Sue Richards

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification

Effect of Healing on the Expression of Transforming Growth Factor βs and their Receptors in Chronic Venous Leg Ulcers Allison J. Cowin, Nicholas Hatzirodos,

Tissue proteomics: a new investigative tool for renal biopsy analysis

Presentation transcript:

Quantitative Amplification of Genomic DNA from Histological Tissue Sections after Staining with Nuclear Dyes and Laser Capture Microdissection Torsten Ehrig, Sarki A. Abdulkadir, Suzanne M. Dintzis, Jeffrey Milbrandt, Mark A. Watson The Journal of Molecular Diagnostics Volume 3, Issue 1, Pages 22-25 (February 2001) DOI: 10.1016/S1525-1578(10)60645-9 Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 DNA recoveries evaluated by TaqMan PCR. Staining procedures: NS, no stain; TB, toluidine blue O; AB, azure B; MG, methyl green; H, Mayer's hematoxylin. Tissue was sampled by manual gross dissection from histological slides (A and B) or by LCM (C and D). The original tissue samples were formalin-fixed, paraffin-embedded archival surgical pathology tissue (A and C) or unfixed, frozen surgical tumor material (B and D). The y-axis gives the amount of template DNA as determined by TaqMan PCR of genomic β-actin DNA. The indicated amount of DNA refers either to a sampled histological tissue section approximately 1 cm2in area (A and B) or to tissue sampled by 500 LCM firings (C and D). Each data point is the average from two independently processed histological slides. The difference between NS and TB, AB, or MG in B is statistically significant (P < 0.05). The Journal of Molecular Diagnostics 2001 3, 22-25DOI: (10.1016/S1525-1578(10)60645-9) Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions