Survival and death-promoting events after transient spinal cord ischemia in rabbits: Induction of Akt and caspase3 in motor neurons Masahiro Sakurai,

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Presentation transcript:

Survival and death-promoting events after transient spinal cord ischemia in rabbits: Induction of Akt and caspase3 in motor neurons Masahiro Sakurai, MD, PhDa, Tatsuya Nagata, MD, PhDb, Koji Abe, MD, PhDc, Takashi Horinouchi, MD, PhDd, Yasuto Itoyama, MD, PhDb, Koichi Tabayashi, MD, PhDa The Journal of Thoracic and Cardiovascular Surgery Volume 125, Issue 2, Pages 370-377 (February 2003) DOI: 10.1067/mtc.2003.112 Copyright © 2003 American Association for Thoracic Surgery Terms and Conditions

Tabayashi and Sakurai (left to right) The Journal of Thoracic and Cardiovascular Surgery 2003 125, 370-377DOI: (10.1067/mtc.2003.112) Copyright © 2003 American Association for Thoracic Surgery Terms and Conditions

Fig. 1 Histologic findings of the spinal cord after 15 minutes of ischemia and staining with HE. Spinal cords of sham control animals (S; A) and those at 2 days after ischemia (B) showed no histologic changes. At 7 days after ischemia (C), however, motor neurons were selectively lost, without apparent gliosis or cellular infiltration (bar = 100 μm). S, Sham operated. The Journal of Thoracic and Cardiovascular Surgery 2003 125, 370-377DOI: (10.1067/mtc.2003.112) Copyright © 2003 American Association for Thoracic Surgery Terms and Conditions

Fig. 2 Representative Western blot for Akt (upper), caspase3 (middle), and β-actin (bottom). Immunoreactivity was not (Akt and caspase3) detected in sham control animals (S). Strong bands became observed at 8 hours (8 h) after blood flow restoration. The band of Akt became scarcely detectable at 1 day; that of caspase3 became detectable at 1 day after reperfusion and was almost lost at 2 days. β-Actin showed no change. The Journal of Thoracic and Cardiovascular Surgery 2003 125, 370-377DOI: (10.1067/mtc.2003.112) Copyright © 2003 American Association for Thoracic Surgery Terms and Conditions

Fig. 3 Immunostaining against Akt and caspase3 in motor neurons in a sham control spinal cord (A and E) and at 8 hours (B and F), 1 day (C and G) and 2 days (D and H) of reperfusion. Arrowheads show motor neurons that express immunoreactive Akt (B) and caspase3 (F and G), respectively (bar = 100 μm). S, Sham operated. The Journal of Thoracic and Cardiovascular Surgery 2003 125, 370-377DOI: (10.1067/mtc.2003.112) Copyright © 2003 American Association for Thoracic Surgery Terms and Conditions

Fig. 4 Colocalization of Akt and caspase3 in motor neurons at 8 hours after ischemia. Akt was detected by means of fluorescein isothiocyanate staining (green; A) and caspase3 by means of Texas red staining (red; B). The merged image is shown in C, with double-positive regions staining a yellow color (bar = 50 μm). The Journal of Thoracic and Cardiovascular Surgery 2003 125, 370-377DOI: (10.1067/mtc.2003.112) Copyright © 2003 American Association for Thoracic Surgery Terms and Conditions