Polymerase Chain Reaction Detection of Kaposi's Sarcoma-Associated Herpesvirus- Optimized Protocols and Their Application to Myeloma Langxing Pan, Laura.

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Presentation transcript:

Polymerase Chain Reaction Detection of Kaposi's Sarcoma-Associated Herpesvirus- Optimized Protocols and Their Application to Myeloma Langxing Pan, Laura Milligan, Joseph Michaeli, Ethel Cesarman, Daniel M. Knowles The Journal of Molecular Diagnostics Volume 3, Issue 1, Pages 32-38 (February 2001) DOI: 10.1016/S1525-1578(10)60647-2 Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Sensitivity tests of the KSHV primer sets. Various amounts (10, 1, 0.1, 0.01, 0.001, 0.0001, 0.00001, and 0.000001 ng) of DNA from the KSHV-infected BC3 cell line (10–20 copies of the virus per cell) were mixed with 100 ng of HL60 cell line DNA. Each primer set was separately tested on these mixed DNA samples. ORF26nest, nested PCR with ORF26out as outer primer set and ORF26in as inner primer set. The Journal of Molecular Diagnostics 2001 3, 32-38DOI: (10.1016/S1525-1578(10)60647-2) Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Selected samples from PCR validation tests with all of the KSHV and EBV-W fragment primer sets. K1–4, Kaposi's sarcoma cases 1–4; P1,2, Primary effusion lymphoma cases 1 and 2; C2, Negative control tonsil DNA; C3, Negative control Raji cell line DNA; FL1,5, Follicular lymphoma cases 1 and 5. The Journal of Molecular Diagnostics 2001 3, 32-38DOI: (10.1016/S1525-1578(10)60647-2) Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions