Spotting pattern of Arabidopsis protein microarrays.

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Spotting pattern of Arabidopsis protein microarrays. Spotting pattern of Arabidopsis protein microarrays. Two identical fields were spotted per microarray. Each field consists of 16 blocks with an 11 × 11 pattern. Positions of the guide dots are marked by filled circles. Guide dots (mixture of MBP (2,000 ng/μl) and mouse anti-RGS-His6 antibody (1:10)) were spotted in every block six times in an asymmetric fashion to assure correct orientation of each image for analysis and to arrange the grids used for quantification. One position per block was not addressed as indicated by a hyphen. Tanja Feilner et al. Mol Cell Proteomics 2005;4:1558-1568 The American Society for Biochemistry and Molecular Biology